Intraperitoneal Sensitization
Intraperitoneal Sensitization - Related Videos
09:19
Intraperitoneal Injection into Adult Zebrafish
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Cited by 238 •
2010
We demonstrate intraperitoneal injection into adult zebrafish. We use a 10 μl NanoFil microsyringe controlled by a Micro4 controller and UltraMicroPump III. This demonstration includes the use of cold water as an anesthetic.
02:25
Synergistic Effect of Intracranial and Intraperitoneal Delivery of Analgesic Drugs on Thermal Pain Response in a Rat
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2025
Source: Martinez, E., et al. Intracranial Pharmacotherapy and Pain Assays in Rodents. J. Vis. Exp. (2019).This video demonstrates the intracranial administration of an analgesic drug in an anesthetized rat with a pre-implanted guide cannula in the prefrontal cortex. An additional analgesic drug is co-administered intraperitoneally. Following recovery, the rat’s thermal pain response is assessed using Hargreave’s apparatus, where paw withdrawal latency is measured to evaluate the analgesic...
10:30
Dye-sensitized Solar Cells
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2023
Source: Tamara M. Powers, Department of Chemistry, Texas A&M University Today's modern world requires the use of a large amount of energy. While we harness energy from fossil fuels such as coal and oil, these sources are nonrenewable and thus the supply is limited. To maintain our global lifestyle, we must extract energy from renewable sources. The most promising renewable source, in terms of abundance, is the sun, which provides us with more than enough solar energy to fully fuel our...
09:22
Monitoring Immune Cells Trafficking Fluorescent Prion Rods Hours after Intraperitoneal Infection
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Cited by 6 •
2010
Here we describe a novel assay for monitoring prion uptake and trafficking by immune cells immediately following intraperitoneal inoculation by purifying and fluorescently labeling aggregated prion rods from infected brain material then monitoring their uptake and movement from the injection site and characterizing the cells mediating these events.
03:20
Intraperitoneal Injection of Cells: A Method of Delivering Cancer Cells into the Peritoneal Adipose Tissue of Murine Model
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2023
In this video, we perform an intraperitoneal injection of ovarian cancer cell suspension in a mouse model to study the colonization of ovarian cancer cells in peritoneal adipose tissues and their subsequent...
FAQs
After antigen enters the peritoneal cavity, peritoneal immune cells can take it up and present antigen-derived information to lymphocytes. This interaction connects local antigen handling with activation of antigen-specific T-cell and B-cell responses. The resulting cellular coordination helps explain how an exposure in an abdominal compartment can produce broader immune consequences.
Repeated antigen exposure can move the response beyond an initial encounter. With successive exposures, antigen-specific T cells, B cells, and antibodies may be generated, creating a heightened reaction when the animal later encounters the same challenge. This feature allows investigators to examine how prior exposure changes subsequent immune responses.
Because the antigen is introduced into the peritoneal cavity, the method emphasizes immune events associated with an abdominal exposure while also allowing study of systemic responses. Comparing results with other exposure routes can reveal how route influences immune activation and the character of later responses, rather than treating all antigen encounters as equivalent.
A typical experimental sequence begins with antigen delivery into the peritoneal cavity, followed by planned repeated exposures that establish sensitization. Researchers then evaluate the response to a later challenge, focusing on antigen-specific immune activation. The sequence links exposure history to changes in T cells, B cells, antibodies, hypersensitivity, or inflammation.
Within immunology and infection, this approach can model host responses to experimental antigens or infectious agents. It is especially useful for examining immune activation, hypersensitivity, and inflammation in relation to exposure history. These models help investigators connect antigen delivery with the way an animal responds to a later challenge.
Researchers can use the sensitized state to test how vaccines, adjuvants, or immunomodulatory treatments affect antigen-specific responses. The relevant comparison is not limited to initial activation; it can include the heightened response observed after later challenge. This makes the method useful for studying interventions that alter host immune responses.