Kidney Mononuclear Cells
Kidney Mononuclear Cells - Related Videos
06:56
Isolation of Double Negative αβ T Cells from the Kidney
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Cited by 26 •
2014
DNabT cells are rare among peripheral T cells; however, they are abundant in certain non-lymphoid tissues. Difficulty of isolating DN T cells from non-lymphoid tissue hinders their functional analysis despite increasing recognized pathophysiologic significance. We describe a novel protocol for isolation of highly purified DN T cells from murine kidney.
04:11
Mononuclear Cell Isolation from Mouse Brain: A Density Gradient Centrifugation Technique to Isolate Brain Resident Mononuclear Cells
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2023
In this video, we perform the isolation of mononuclear cells from a mouse’s brain using density gradient centrifugation. The isolated mononuclear cell suspension can be used for further downstream analysis.
03:14
Ex Vivo Expansion of Natural Killer Cells from Peripheral Blood Mononuclear Cells
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2025
This video demonstrates a technique for the expansion of natural killer (NK) cells directly from peripheral blood mononuclear cells (PBMCs) using a feeder cell line. PBMCs are co-cultured with genetically modified lymphoblastoid feeder cells expressing membrane-bound interleukin-21 (mIL-21), and the culture is supplemented with the cytokines interleukin-2 (IL-2) and interleukin-15 (IL-15). Together, this cocktail of ligands leads to the prolonged expansion of NK cells with high purity.
03:25
Culturing of Activated T Cells from Human Peripheral Blood Mononuclear Cells
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2025
In this video, we describe a protocol for human primary T cell activation. The incubation of naïve T cells with magnetic beads coated with anti-CD3 and anti-CD28 antibodies, followed by interleukin-2 or interleukin-15 treatment, leads to the activation of naïve T cells and their differentiation and proliferation into effector or memory T cells.
05:01
Lamina Propria Mononuclear Cell Isolation: A Silica-based Density Gradient Centrifugation Technique to Obtain Mononuclear Cells from Murine Colon
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2023
In this video, we demonstrate the isolation of lamina propria mononuclear cells from murine colonic tissue by enzymatic digestion using collagenase E and silica-based density separation media gradient separation. The isolated mononuclear cells can be used for subsequent downstream analysis.
FAQs
Different cell types within the population can contribute distinct immune functions. Lymphocytes, monocytes, macrophages, and dendritic cells may differ in how they detect threats, communicate through cytokines, and influence inflammation. Separating these cellular contributions helps researchers determine whether a renal response reflects antigen-driven activity, innate sensing, tissue defense, or inflammatory regulation.
These recognition systems provide complementary ways to detect danger in renal tissue. Antigen receptors support responses directed toward specific antigens, whereas innate immune sensors detect broader danger signals. Their activation can influence cytokine release and communication between cells, shaping how local immune responses develop during infection or tissue injury.
Each condition can change the signals encountered by kidney mononuclear cells and thereby influence their phenotype and function. Pathogens may stimulate host defense, tissue injury may generate danger signals, and inflammatory disease may sustain cell-to-cell communication and cytokine production. Examining these changes helps connect local immune activity with kidney pathology.
A study generally begins by obtaining or isolating the cells from renal tissue, followed by examining their phenotypes or functions. Researchers can then interpret the observed immune characteristics in relation to local defense, inflammation, infection, or injury. This workflow links cellular observations to broader questions about renal immunity and disease mechanisms.
They are especially useful when investigators need to examine immune activity within the kidney rather than relying only on systemic measurements. Their analysis can clarify host-pathogen interactions, reveal how renal tissue responds to inflammatory disease or injury, and identify cellular behaviors that may be relevant to local immune regulation.
Analysis can reveal which cellular phenotypes and functions are associated with renal defense, inflammation, or disease-related responses. These findings may improve understanding of host-pathogen interactions and help identify immune pathways or cell behaviors that could serve as potential therapeutic targets. The value lies in connecting cellular properties with tissue-level immune outcomes.