Murine Cd40-b Cells
Murine Cd40-b Cells - Related Videos
12:24
Murine Model of CD40-activation of B cells
0 Views •
Cited by 13 •
2010
In this video, we demonstrate the procedure of CD40-activation and expansion of murine B cells from splenocytes of C57BL/6 mice, which can be used as a model antigen-presenting cell (APC) to study induction of immunity.
13:27
Generation of Human CD40-activated B cells
0 Views •
Cited by 36 •
2009
In this video we present the ex vivo generation and expansion of human CD40-activated B cells (CD40-B) from peripheral blood mononuclear cells (PBMC) by stimulation with CD40 ligand and interleukin-4.
08:56
Isolation of Murine Embryonic Hemogenic Endothelial Cells
0 Views •
Cited by 11 •
2016
Hematopoietic stem and progenitor cells (HSPC) derive from specialized (hemogenic) endothelial cells during development, yet little is known about the process by which some endothelial cells specify to become blood forming. We demonstrate a flow-cytometry based method allowing simultaneous isolation of hemogenic endothelial cells and HSPC from murine embryonic tissues.
01:31
Magnetic Hyperthermia-Induced Cell Damage in Murine Microglial Cells
0 Views •
2025
Take adhered murine microglial cells in a multiwell plate.Add polyacrylic acid-coated magnetic nanoparticles (MNPs) to the test well, leaving another well untreated as a control.Incubate the cells to allow MNP uptake via endocytosis, enabling their internalization.Wash with buffer to remove unbound MNPs.Add an enzyme solution to detach the cells.Transfer the detached cells to tubes and centrifuge to pellet them.Discard the supernatant, resuspend the pellet in basal media, and transfer the...
08:42
Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells
0 Views •
Cited by 14 •
2018
The goal of this study was to formulate technologies that allow for successful gene transduction in primary natural killer (NK) cells. The dextran-mediated lentiviral transduction of human or mouse primary NK cells results in higher gene expression efficiencies. This method of gene transduction will vastly improve NK cell genetic manipulation.
FAQs
CD40 engagement activates intracellular signaling programs that alter several B-cell functions at once. The resulting changes support survival and proliferation, while also increasing the cells’ capacity to present antigen. This combination makes Murine CD40-B cells useful for examining the consequences of CD40 pathway activation rather than treating antibody production as an isolated endpoint.
CD40L serves as the ligand that engages the CD40 pathway on B cells. That interaction provides the initiating signal for downstream activation, survival, proliferation, and enhanced antigen-presenting capacity. In immunology experiments, examining these outcomes helps reveal how a T-cell-associated signal influences B-cell behavior and subsequent immune responses.
These signals influence antibody responses by changing the state and function of B cells rather than acting only as a growth cue. Because activation also affects antigen presentation and survival, CD40 engagement can modify communication within the immune response. Studying these linked effects helps identify mechanisms that support protective immunity or contribute to dysregulated responses.
Pathogens can be studied in relation to B-cell changes produced by CD40 signaling, including altered activation, survival, proliferation, and antigen presentation. The model connects a defined immune pathway with broader questions about how B cells communicate with T cells during infection. It can also help examine mechanisms associated with effective protection or immune dysregulation.
Researchers use the cells as an experimentally tractable system to examine B-cell function under CD40 pathway activation. Studies can focus on B-cell interactions with T cells, changes in antigen-presenting capacity, antibody-response mechanisms, or responses relevant to pathogens. The specific readout depends on whether the experiment targets cellular activation, immune regulation, or host-pathogen biology.
Assessment can extend to survival, antigen-presenting capacity, interactions with T cells, antibody responses, and immune regulation. Considering these outcomes together is important because CD40 signaling produces coordinated changes rather than a single response. This broader profile allows investigators to relate cellular behavior to protective immunity, pathogen-associated processes, or dysregulated immune responses.
It is especially relevant when an intervention is expected to alter CD40-linked B-cell behavior or communication within the immune response. Investigators can use the system to define how modulation affects activation, survival, proliferation, antigen presentation, or antibody-related mechanisms. Results may clarify whether a strategy promotes protective responses or shifts immunity toward dysregulation in infection-related settings.