Rbl-2h3 Cells
Rbl-2h3 Cells - Related Videos
17:35
A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent
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Cited by 24 •
2013
Mast cell degranulation, the release of allergic mediators, is important in allergy, asthma, and parasite defense. Here we demonstrate techniques1 for assessing effects of drugs and toxicants on degranulation, methodology recently utilized to exhibit the powerful inhibitory effect of antibacterial agent triclosan2.
13:45
Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin
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Cited by 7 •
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This paper describes the methodology to determine the chemotactic response of leukocytes to specific ligands and identify interactions between the cell surface receptors and cytosolic proteins using live cell imaging techniques.
03:34
An In Vitro Technique to Visualize Exocytosis of Secretory Granules in Mast Cells
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2025
This video demonstrates a technique to visualize exocytosis in mast cells. The cells are incubated with FITC-dextran — a fluorescent reporter — which is taken up by cells inside secretory granules (SGs), where the acidic pH quenches its fluorescence. Upon stimulation of granule exocytosis, the medium's higher pH induces reporter fluorescence inside the fused SGs, which is visualized using a fluorescence microscope.
05:39
Humanized Mediator Enzyme Release Assay: An Immunochemical Method to Screen Allergic Potential of Test Antigen by Monitoring Basophil Degranulation Response In Vitro
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2025
In this video, we describe an in vitro method to evaluate the allergic potential of an antigen to induce basophil degranulation. β-hexosaminidase is used as an index of chemical mediator release, and the enzyme activity of β-hexosaminidase is measured using a fluorogenic substrate.
10:04
Tractable In Vivo Reprogramming of Tumor Cells to Type 1 Conventional Dendritic Cell-like Cells
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2025
This protocol describes the in vivo reprogramming of mouse cancer cells into type 1 dendritic-like cells within the tumor microenvironment through enforced expression of the transcription factors PU.1, IRF8, and BATF3.
FAQs
Antigen cross-linking of receptor-bound IgE brings FcεRI-associated receptors into an activating arrangement. This initiates intracellular signaling, which connects receptor engagement to granule exocytosis. The released mediators provide a functional readout of activation, allowing investigators to relate an external antigen stimulus to a measurable cellular response in vitro.
FcεRI signaling is the link between recognition and secretion. Once receptor-bound IgE is cross-linked, intracellular events activate the cell’s secretory machinery, producing granule exocytosis. In RBL-2H3 experiments, this relationship lets researchers examine how antibodies, allergens, or immune-modulating compounds alter receptor-triggered activation rather than measuring mediator release as an isolated endpoint.
Histamine and β-hexosaminidase release serve as measurable indicators of granule exocytosis after FcεRI activation. Measuring these mediators allows investigators to convert a receptor-triggering event into an assay outcome that can be compared across experimental conditions. This makes the system useful for quantifying degranulation and assessing whether a tested substance changes activation.
Consistent responses make this model practical for comparing different antigens, antibodies, allergens, or immune-modulating compounds. Because the same receptor-linked activation pathway produces measurable mediator release, researchers can focus on differences between test conditions. The resulting data help investigate immediate hypersensitivity and the cellular events associated with inflammatory responses.
A conceptual workflow begins with receptor-bound IgE on the cells, followed by exposure to an antigen that cross-links FcεRI. Researchers then assess granule exocytosis or mediator release, commonly using histamine and β-hexosaminidase as outcomes. This sequence connects the initiating stimulus with a quantifiable degranulation response.
Within immunology and infection research, these cells support analysis of Fc receptor signaling, immediate hypersensitivity, and mast cell contributions to inflammatory and host-defense responses. They also provide a testing platform for allergens, antibodies, and other immune-modulating compounds. Results can show whether a stimulus promotes or alters mediator release associated with cellular activation.