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JoVE Journal
Immunology and Infection

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A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation
 

A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent

Article doi: 10.3791/50671
November 1st, 2013

Summary November 1st, 2013

Mast cell degranulation, the release of allergic mediators, is important in allergy, asthma, and parasite defense. Here we demonstrate techniques1 for assessing effects of drugs and toxicants on degranulation, methodology recently utilized to exhibit the powerful inhibitory effect of antibacterial agent triclosan2.

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