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A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent

A Microplate Assay to Assess Chemical Effects on RBL-2H3 Mast Cell Degranulation: Effects of Triclosan without Use of an Organic Solvent

DOI:
10.3791/50671-v

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17:35 min

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November 01, 2013

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, , ,
1Department of Molecular and Biomedical Sciences,University of Maine, Orono, 2Graduate School of Biomedical Sciences and Engineering,University of Maine, Orono

Chapters

  • 00:05Title
  • 02:45Preparation of Cells
  • 04:06Preparation of Triclosan (TCS)
  • 06:12Antigen-stimulated Degranulation Assay Using RBL-2H3 Cells
  • 12:13Results: TCS Causes a Significant Dose-responsive Inhibition of Mas Cell Degranulation
  • 16:38Conclusion

Summary

Automatic Translation

Automatic Translation

Mast cell degranulation, the release of allergic mediators, is important in allergy, asthma, and parasite defense. Here we demonstrate techniques1 for assessing effects of drugs and toxicants on degranulation, methodology recently utilized to exhibit the powerful inhibitory effect of antibacterial agent triclosan2.

Tags

Keywords: Microplate AssayRBL-2H3 Mast CellsMast Cell DegranulationTriclosanAllergic Mediatorsβ-hexosaminidaseHigh-throughput ScreeningOrganic Solvent-freeUV-Vis Spectrophotometry

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