Electron Beam Scanning
Electron Beam Scanning - Related Videos
08:57
Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue
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Cited by 105 •
2011
This protocol describes how resin embedded brain tissue can be prepared and imaged in the three dimensions in the focussed ion beam, scanning electron microscope.
01:21
Focused Ion Beam Scanning Electron Microscopy for 3D Reconstruction of Synaptically Linked Neurons
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2025
Take a fixed section of mouse cortical brain tissue exhibiting neuronal axons and dendrites. The dendrites possess protrusions, known as dendritic spines, which form synapses with axons.Incubate the tissue with osmium-ferrocyanide, which binds to lipids, followed by another osmium application, which enhances structural contrast for imaging.Stain with a heavy metal compound that binds to macromolecules and enhances contrast.Dehydrate using increasing alcohol concentrations for resin...
11:41
Scanning Electron Microscopy (SEM)
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2023
Source: Laboratory of Dr. Andrew J. Steckl — University of Cincinnati A scanning electron microscope, or SEM, is a powerful microscope that uses electrons to form an image. It allows for imaging of conductive samples at magnifications that cannot be achieved using traditional microscopes. Modern light microscopes can achieve a magnification of ~1,000X, while typical SEM can reach magnifications of more than 30,000X. Because the SEM doesn’t use light to create images, the resulting pictures it...
07:00
Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography
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Cited by 8 •
2021
Using focused ion beam milling to produce vitreous on-grid lamellae from plunge frozen biological samples for cryo-electron tomography.
10:25
Single-Digit Nanometer Electron-Beam Lithography with an Aberration-Corrected Scanning Transmission Electron Microscope
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Cited by 4 •
2018
We use an aberration-corrected scanning transmission electron microscope to define single-digit nanometer patterns in two widely-used electron-beam resists: poly (methyl methacrylate) and hydrogen silsesquioxane. Resist patterns can be replicated in target materials of choice with single-digit nanometer fidelity using liftoff, plasma etching, and resist infiltration by organometallics.
FAQs
Magnetic deflection changes the path of the focused electron beam, sweeping it across different positions on a stationary tungsten target. Each position can contribute to X-ray production, allowing the system to sample the target rapidly without moving the target itself. This beam-steering mechanism is central to acquiring medical images quickly, particularly when anatomy changes during the scan.
Because the X-ray tube does not need to rotate mechanically, the system can move the beam across the target with very short exposure times. Rapid acquisition helps record motion-sensitive anatomy before cardiac movement substantially changes the image. In practice, this can reduce motion-related artifacts and support clearer evaluation of structures affected by the heartbeat.
After X-rays pass through the body, detectors record the transmitted radiation. The collected measurements are then used to reconstruct cross-sectional images, converting differences in detected radiation into a view of internal structures. Thus, the detector stage is not simply a display step; it supplies the measurements needed for image formation and interpretation.
During an examination, a focused electron beam is directed toward the tungsten target while magnetic deflection sweeps it across the target. The generated X-rays pass through the body and reach detectors, which collect the imaging data. A reconstruction step then produces cross-sectional views. The sequence is designed to complete image acquisition rapidly rather than rely on mechanical tube rotation.
It can support cardiac assessment by producing rapid images of the region containing the coronary arteries. Short exposure times help limit blurring from cardiac motion, allowing clinicians to evaluate coronary artery calcification in cross-sectional images. The technique is therefore relevant when the imaging target moves and when timing the acquisition is important to preserving interpretable anatomical detail.
The method provides cross-sectional visualization of internal structures and is especially relevant to anatomy that is difficult to capture while still. In medicine, its documented use includes cardiac imaging, assessment of coronary artery calcification, and visualization of motion-sensitive anatomy. The principal outcome is rapidly acquired image data that can be reconstructed for evaluation of these structures.