Mammary Tissue Dissociation
Mammary Tissue Dissociation - Related Videos
05:26
Mechanical Dissociation: A Method to Obtain Viable Cells from a Tissue
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2023
This video describes the non-enzymatic dissociation of fresh human tissue to obtain viable cells. The technique is used for qualitative and quantitative analysis of CD45 positive cells (lymphocytes/leukocytes) present in various normal and malignant human tissues.
03:35
Dissociating and Culturing Neurons from Hippocampal Tissue Samples
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2025
This video demonstrates the dissociation and culture of hippocampal neurons from hippocampal tissue samples using enzymatic and mechanical dissociations. The treatment with Ara-C inhibits the growth of non-neuronal cells, facilitating the selective growth of hippocampal neurons.
06:30
Intraductal Delivery to the Rabbit Mammary Gland
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Cited by 9 •
2017
Here, we describe a technique for the localized delivery of reagents to the rabbit mammary gland via an intraductal injection. In addition, we describe a protocol for visualization and the confirmation of delivery by high-resolution ultrasound imaging of contrast agents.
09:59
Transfer of Mammary Gland-forming Ability Between Mammary Basal Epithelial Cells and Mammary Luminal Cells via Extracellular Vesicles/Exosomes
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Cited by 8 •
2017
This protocol describes methods for purifying, quantitating, and characterizing extracellular vesicles (EVs)/exosomes from non-adherent/mesenchymal mammary epithelial cells and for using them to transfer mammary gland-forming ability to luminal mammary epithelial cells. EVs/exosomes derived from stem-like mammary epithelial cells can transfer this cell property to cells that ingest the EVs/exosomes.
07:03
Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator
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Cited by 45 •
2009
Dissociating cells from specific tissue types requires specific parameters for tissue agitation to obtain a high volume of viable, culturable cells. The Miltenyi gentleMACS dissociator optimizes this task with a simple, practical protocol. In this publication the use of this apparatus on lung tissue is explained.
FAQs
Collagenase breaks down extracellular matrix proteins that hold mammary cells within the tissue structure. Mechanical mincing first increases the exposed tissue surface, allowing enzymatic digestion to act more effectively. Together, these steps reduce structural complexity while helping retain viable epithelial, stromal, and immune cell populations for downstream analysis.
These processing choices help enrich different components after digestion. Filtration separates material according to physical size, centrifugation supports separation of cellular fractions, and selective culture favors the growth or maintenance of chosen populations. The resulting preparation can therefore be adjusted toward epithelial, stromal, or immune cells, depending on the intended experiment.
Single-cell preparations are useful when researchers need to examine individual cells with flow cytometry or molecular profiling. Organoid-enriched preparations better retain aspects of three-dimensional tissue organization and can support studies of tissue structure or ex vivo function. Choosing between them depends on whether the experiment prioritizes cellular composition or organized growth.
A typical workflow begins by mechanically mincing the breast tissue, followed by enzymatic digestion with an enzyme such as collagenase. The digested material then undergoes filtration or centrifugation, and selective culture may further enrich chosen populations. The final preparation can be used as single cells or as an organoid-enriched sample for downstream assays.
The resulting preparations support microscopy, flow cytometry, molecular profiling, and ex vivo functional assays. Microscopy can examine cellular or organoid organization, while flow cytometry helps characterize enriched populations. Molecular and functional approaches provide complementary information about cell states, tissue behavior, and responses under controlled experimental conditions.
In medicine, the method supports investigations of mammary development, cancer biology, tissue organization, and treatment response. By reducing the complexity of intact tissue while preserving relevant cell populations, researchers can compare cellular behavior, examine structural relationships, and test responses in ex vivo systems that are more experimentally accessible than the original tissue.