Pediatric Spine Tumor
Pediatric Spine Tumor - Related Videos
09:43
Transplantation of Zebrafish Pediatric Brain Tumors into Immune-competent Hosts for Long-term Study of Tumor Cell Behavior and Drug Response
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Cited by 22 •
2017
The transplantation of cancer cells is an important tool for the identification of cancer mechanisms and therapeutic responses. Current techniques depend on immune-incompetent animals. Here, we describe a method to transplant zebrafish tumor cells into immune-competent embryos for the long-term analysis of tumor cell behavior and in vivo drug responses.
04:27
Co-culturing Glutamatergic Neurons and Pediatric High-Grade Glioma Cells
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2025
This video showcases a technique for co-culturing glutamatergic neurons and pediatric high-grade glioma (pHGG) cells within a microfluidic device. Human induced pluripotent stem cells are introduced into the microfluidic system and incubated to promote their differentiation into mature cortical glutamatergic neurons. Following this, the pHGG cells are introduced onto the mature neurons, establishing a co-culture and enabling interactions between neurons and pHGG cells.
02:49
Harvesting DRG from Rat Spine: A Procedure to Extract Dorsal Root Ganglion from the Spine of a Rat Model
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2023
In this video, we demonstrate the extraction of dorsal root ganglion or DRG from a harvested rat spine for downstream applications.
05:25
Quantification of Dendritic Spines Using Confocal Microscopy Imaging
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2025
Source: Gouder, et. al. Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells. J. Vis. Exp. (2015).This video demonstrates the imaging and analysis of dendritic spines in transduced, immunolabeled pyramidal neurons using confocal microscopy. Background noise is reduced, and dendrites are traced by estimating their diameter and length. Automated spine segmentation involves setting parameters for dendrite and spine dimensions...
05:28
A Mouse Model of Lumbar Spine Instability
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Cited by 14 •
2021
We developed a lumbar intervertebral disc degeneration mouse model by resection of L3–L5 spinous processes along with supra- and inter-spinous ligaments and detachment of paraspinous muscles.
FAQs
Symptoms reflect more than the presence of a lesion; they depend on how its location, size, and growth rate affect nearby structures. Pressure on the spinal cord or nerve roots may alter neurological function, while involvement of vertebrae or surrounding tissues can affect spinal stability. This variability explains why children with similar lesions may have different clinical findings.
An important clinical distinction is whether a lesion mainly threatens neural tissue, spinal support, or both. Effects on the spinal cord and nerve roots can influence neurological function, whereas vertebral involvement may compromise stability. Considering these relationships helps clinicians interpret symptoms alongside imaging and plan management that addresses function as well as the structural condition.
Growth rate changes how the lesion may present and how its effects are interpreted. A larger mass or faster expansion can influence the degree of compression, tissue disruption, or instability, but symptoms still depend on location. Clinicians therefore combine the neurological examination with imaging rather than using one feature alone to characterize the child’s condition.
Assessment usually begins with a neurological examination and magnetic resonance imaging. The examination documents neurological function, while MRI shows the lesion’s relationship to the spinal cord, nerve roots, vertebrae, and surrounding tissues. When imaging and clinical findings require further characterization, tissue analysis can add diagnostic information that supports a more specific management plan.
Tissue analysis is used when additional characterization of a lesion is needed, rather than as a substitute for the clinical and imaging assessment. By helping identify what the lesion represents, it can inform decisions about surgery, radiation, or systemic treatment. This diagnostic step is especially relevant when treatment planning must balance disease control with preservation of developing spinal and neurological function.
Management planning must account for both the lesion and the child’s long-term development. Depending on the diagnostic findings, care may involve surgery, radiation, systemic treatment, or combinations of these approaches. Follow-up monitoring is important because clinicians assess neurological function, spinal development, mobility, and quality of life over time, not only the immediate response to treatment.