Point Of Sickling
Point Of Sickling - Related Videos
05:23
Continuous Manual Exchange Transfusion for Patients with Sickle Cell Disease: An Efficient Method to Avoid Iron Overload
0 Views •
Cited by 4 •
2017
We have outlined a method of continuous manual exchange transfusion for the treatment of sickle cell disease in patients. This safe protocol was designed to effectively limit iron overload in patients in need of chronic transfusions and can be used extensively without any special equipment.
08:23
Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry
0 Views •
Cited by 16 •
2019
Here, we present oxygen gradient ektacytometry, a rapid and reproducible method to measure red blood cell deformability in samples from patients with sickle cell disease under controlled deoxygenation and reoxygenation. This technique provides a way to study red blood cell sickling and to monitor sickle cell disease treatment efficacy.
09:10
Synthesis of Masarimycin, a Small Molecule Inhibitor of Gram-Positive Bacterial Growth
0 Views •
2022
A detailed protocol is presented for preparing the bacteriostatic diamide masarimycin, a small molecule probe that inhibits the growth of Bacillus subtilis and Streptococcus pneumoniae by targeting cell wall degradation. Its application as a chemical probe is demonstrated in synergy/antagonism assays and morphological studies with B. subtilis and S. pneumoniae.
07:42
Magnetic Levitation Coupled with Portable Imaging and Analysis for Disease Diagnostics
0 Views •
Cited by 11 •
2017
We present a magnetic levitation technique coupled with automated imaging and analysis in both a smartphone-compatible device and a device with embedded imaging and processing. This is applied to measure the density distribution of cells with two demonstrated biomedical applications: sickle cell disease diagnosis and separating white and red blood cells.
11:29
Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents
0 Views •
Cited by 52 •
2015
This video article describes experimental procedures to study long-term plasticity and its associative processes such as synaptic tagging, capture and cross-tagging in the CA1 pyramidal neurons using acute hippocampal slices from rodents.
FAQs
Oxygen tension serves as the controlling condition because the transition begins when hemoglobin S becomes deoxygenated. Under reduced oxygen availability, hemoglobin S molecules associate into rigid polymers. This molecular change precedes visible cellular distortion, so the measured threshold links an environmental variable, oxygen level, to the onset of red-cell behavior relevant to sickle cell disease.
Polymer formation changes red-cell mechanics rather than merely altering appearance. The resulting rigid structures distort cells and reduce their flexibility, which can make movement through small blood vessels more difficult. This connection explains why Point Of Sickling is biologically informative: it links hemoglobin S behavior to cellular properties that may contribute to vascular obstruction and tissue hypoxia.
Point Of Sickling measurements can help investigate how hemoglobin S behaves in different biological situations. The measurement is relevant to disease severity, hemoglobin variants, and antisickling treatments. Its value therefore extends beyond detecting sickling: it supports comparisons of how these factors relate to the oxygen conditions associated with the process.
Laboratory assessment relates oxygen availability to the appearance of sickling in blood containing sickle hemoglobin. The oxygen tension associated with that transition is recorded as the point of sickling. This approach characterizes sickling tendency under low-oxygen conditions and provides information that cannot be obtained solely from observing clinical manifestations.
Researchers can use the measurement when investigating hemoglobin variants because the assay examines hemoglobin S behavior under low-oxygen conditions. Comparing results across variant-related investigations may clarify how hemoglobin composition relates to sickling tendency. This makes the assessment useful for studying molecular differences that could contribute to variation in sickle cell disease.
The measurement provides context for complications associated with reduced oxygen availability. When polymer formation makes red cells rigid and less flexible, the cells may obstruct small blood vessels, contributing to vaso-occlusion and tissue hypoxia. Point Of Sickling therefore helps connect laboratory observations of hemoglobin S behavior with mechanisms underlying clinically important complications.