Journal
/
Medicine
/
Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal Medicine
Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry

Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry

DOI:
10.3791/60213-v

•

08:23 min

•

November 05, 2019

•

, , , , , ,
1Laboratory of Clinical Chemistry and Hematology, University Medical Center Utrecht,Utrecht University, 2Van Creveldkliniek, University Medical Center Utrecht,Utrecht University, 3Department of Pediatrics, Division of Hematology/Oncology,Baylor College of Medicine

Chapters

  • 00:04Title
  • 01:12Start-up of the Ektacytometer
  • 02:13Sample Collection and Preparation
  • 03:07Oxygen Gradient Ektacytometry Measurement
  • 04:41Cleaning of the Ektacytometer
  • 05:29Shutdown of the Machine
  • 06:02Results: Characterization of Sickling Oxygen Gradient Ektacytometry
  • 07:32Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present oxygen gradient ektacytometry, a rapid and reproducible method to measure red blood cell deformability in samples from patients with sickle cell disease under controlled deoxygenation and reoxygenation. This technique provides a way to study red blood cell sickling and to monitor sickle cell disease treatment efficacy.

Tags

Keywords: Oxygen Gradient EktacytometrySicklingSickle Cell DiseaseFunctional AssayBiomarkerDisease SeverityTreatment EfficacySample PreparationStandardizationDeoxygenationComplete Blood Count

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/60213?article_type=v

Production and Detection of Reactive Oxygen Species (ROS) in Cancers
check_url/60213?article_type=v

Controlled Cervical Laceration Injury in Mice
check_url/60213?article_type=v

Controlled Cortical Impact Model for Traumatic Brain Injury
check_url/60213?article_type=v

Characterization of Metabolic Status in Nonhuman Primates with the Intravenous Glucose Tolerance Test
check_url/60213?article_type=v

Drug-Induced Sleep Endoscopy (DISE) with Target Controlled Infusion (TCI) and Bispectral Analysis in Obstructive Sleep Apnea
check_url/60213?article_type=v

Topical Airway Anesthesia for Awake-endoscopic Intubation Using the Spray-as-you-go Technique with High Oxygen Flow
check_url/60213?article_type=v

Contractions of Human-iPSC-derived Cardiomyocyte Syncytia Measured with a Ca-sensitive Fluorescent Dye in Temperature-controlled 384-well Plates
check_url/60213?article_type=v

Measurement of the Hepatic Venous Pressure Gradient and Transjugular Liver Biopsy
check_url/60213?article_type=v

A Rat Carotid Artery Pressure-Controlled Segmental Balloon Injury with Periadventitial Therapeutic Application
check_url/60213?article_type=v

Quality-Controlled Sputum Analysis by Flow Cytometry

Read Article