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JoVE Journal
Medicine

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Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry
 

Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry

Article DOI: 10.3791/60213-v
November 5th, 2019

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Summary November 5th, 2019

Here, we present oxygen gradient ektacytometry, a rapid and reproducible method to measure red blood cell deformability in samples from patients with sickle cell disease under controlled deoxygenation and reoxygenation. This technique provides a way to study red blood cell sickling and to monitor sickle cell disease treatment efficacy.

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