Ex-vivo Tissue Imaging
Ex-vivo Tissue Imaging - Related Videos
10:35
Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
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2016
The assembly and use of a multimodal microendoscope is described which can co-register superficial tissue image data with tissue physiological parameters including hemoglobin concentration, melanin concentration, and oxygen saturation. This technique can be useful for evaluating tissue structure and perfusion, and can be optimized for individual needs of the investigator.
07:15
Transient Optical Clearing Using Absorbing Molecules for Ex Vivo and In Vivo Imaging
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Cited by 1 •
2025
Optical clearing techniques, such as absorbing molecules solutions, reduce light scattering temporarily to enhance biological imaging and offer biocompatible, reversible in vivo imaging strategies for deeply embedded organs. This protocol presents detailed experimental procedures as well as advanced image processing to achieve high-quality, dynamic imaging for real-time dynamics in living animals.
06:46
Non-invasive In Vivo Fluorescence Optical Imaging of Inflammatory MMP Activity Using an Activatable Fluorescent Imaging Agent
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Cited by 4 •
2017
This paper explains the application of fluorescent imaging using an activatable optical imaging probe to visualize the in vivo activity of key matrix metalloproteinases in two different experimental models of inflammation.
11:51
Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila
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Cited by 9 •
2009
Mesoscopic fluorescence tomography operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The technique is based on multi-projection illumination and a photon transport description. We demonstrate in-vivo whole-body 3D visualization of the morphogenesis of GFP-expressing wing imaginal discs in Drosophila melanogaster.
12:08
Ex Vivo Infection of Live Tissue with Oncolytic Viruses
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Cited by 22 •
2011
Oncolytic viruses are promising for cancer therapeutics. The ability to ascertain the infectability of live tissue specimens obtained from patients prior to treatment is a unique advantage of this therapeutic approach. This protocol describes how to process tissues for ex vivo infection with oncolytic virus and subsequent viral quantification.
FAQs
Maintaining the original arrangement of cells and tissue layers allows researchers to relate molecular signals to specific anatomical locations. In neural samples, this spatial context helps connect fluorescence or morphology with neuronal organization, synaptic architecture, and vascular patterns. The resulting images can therefore show not only whether a feature is present, but where it occurs within the nervous system.
Viable preparations retain tissue activity for examining responses to experimental conditions, whereas fixation stabilizes the removed tissue for labeling and structural visualization. This distinction affects the information available from the sample: viability supports analysis of responses, while fixed and labeled material supports detailed examination of morphology, fluorescence, and organization across defined regions or cellular layers.
Fluorescence reveals labeled molecular signals, morphology shows the form and arrangement of structures, and optical sections provide views through different depths of the specimen. Combining these readouts helps researchers examine cellular layers and regional organization rather than relying on a single surface image. Together, they strengthen analysis of neuronal structure, synaptic architecture, and related tissue patterns.
After tissue removal, researchers can examine neural structures and signals across selected regions or cellular layers with detailed microscopy. This makes subtle patterns in neuronal organization, synaptic architecture, or vascular structure more accessible for analysis than they may be in a living animal. The approach is especially useful when experimental conditions alter tissue structure or molecular labeling.
A typical workflow begins by removing the brain or nervous tissue, then either maintaining it in a viable state or fixing and labeling it, depending on the experimental objective. Researchers next use microscopy to capture fluorescence, morphology, and optical sections across relevant regions or layers. These images are then analyzed for structural organization and experimental responses.
The method is useful when investigators need detailed evidence about neural development, connectivity, disease mechanisms, or responses to drugs and genetic manipulation. Imaging can connect experimental treatment or genotype with changes in neuronal organization, synaptic architecture, vascular patterns, or molecular signals. It therefore provides a spatially resolved way to examine how conditions affect nervous tissue.
Ex vivo analysis can document changes in fluorescence, cellular morphology, optical structure, neuronal organization, synaptic architecture, and vascular patterns. These outcomes help researchers compare tissue from different experimental conditions and determine whether drugs, genetic manipulation, or disease-related processes are associated with altered neural structure or molecular signals. Interpretation remains tied to the specific regions and layers imaged.