Hippocampal Slice Preparation
Hippocampal Slice Preparation - Related Videos
08:20
Organotypic Hippocampal Slice Cultures
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Cited by 52 •
2011
We describe a method to prepare organotypic hippocampal slices that can be easily adapted to other brain regions. Brain slices are laid on porous membranes and culture media is allowed to form an interface. This method preserves the gross architecture of the hippocampus for up to 2 weeks in culture.
09:39
Improved Preparation and Preservation of Hippocampal Mouse Slices for a Very Stable and Reproducible Recording of Long-term Potentiation
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Cited by 20 •
2013
This paper presents a complete methodology to prepare and preserve in vitro acute hippocampal slices from adult mice. This protocol allows the recording of very stable long-lasting long-term potentiation (LTP) for more than 8 hours with a success rate of 95%.
14:57
Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology
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Cited by 62 •
2011
This article outlines procedures for preparing hippocampal slices from rats and transgenic mice for the study of synaptic alterations associated with brain aging and age-related neurodegenerative diseases, such as Alzheimer’s disease.
04:41
Preparation of Oligomeric β-amyloid1-42 and Induction of Synaptic Plasticity Impairment on Hippocampal Slices
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Cited by 74 •
2010
One feature of Alzheimer's Disease is the elevation of Aβ1-42 peptide. Here we provide a protocol for preparing synthetic Aβ1-42 oligomers, which impairs hippocampal Long-Term Potentiation, a cellular correlate of memory. This procedure is useful for investigating mechanisms of Aβ-induced pathology and drug screening.
02:55
Obtaining Horizontal Mouse Hippocampal Brain Slices
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2025
This video demonstrates a detailed protocol for preparing horizontal hippocampal brain slices from mice, focusing on preserving the integrity of hippocampal fiber pathways. The process involves carefully bisecting and securing the brain, using a vibratome for slicing, and maintaining optimal tissue conditions with a carbogenated fluid, resulting in hippocampal brain slices ready for further experimentation.
FAQs
Rapid removal and cooling are preservation steps intended to maintain neuronal viability while tissue is handled outside the brain. Cooling is paired with oxygenated artificial cerebrospinal fluid during vibratome cutting, creating controlled conditions that help retain functional tissue and local synaptic connections for later experiments.
Oxygenated artificial cerebrospinal fluid provides the controlled solution used during cutting, while physiological solution supports subsequent slice maintenance. The preparation links oxygenation and this fluid environment with preservation of neuronal viability and local synaptic connections, allowing researchers to examine activity, synaptic transmission, and plasticity after the tissue has been isolated.
The vibratome cuts cooled brain tissue into thin sections while the tissue is surrounded by oxygenated artificial cerebrospinal fluid. This combination supports preservation of neuronal viability and local synaptic connections. The resulting sections can therefore function as controlled preparations for measurements of hippocampal electrical activity, synaptic transmission, and plasticity.
Electrophysiology, imaging, and pharmacological experiments provide complementary ways to examine the prepared tissue. Electrophysiology can assess electrical activity and synaptic transmission, imaging offers another way to study activity, and pharmacological experiments test responses to drugs. Together, these approaches allow investigators to analyze hippocampal function under controlled experimental conditions.
After sectioning, the slices are maintained in physiological solution before and during experiments. This maintenance step keeps the preparation in the controlled environment needed for later examination. Researchers can then apply electrophysiological, imaging, or pharmacological approaches to investigate electrical activity, synaptic transmission, and plasticity in the preserved tissue.
Hippocampal slices provide a tractable model for studying learning and memory at the level of neural circuits and cellular mechanisms. Because local synaptic connections can remain available for examination under controlled conditions, researchers can investigate electrical activity, synaptic transmission, and plasticity that are relevant to hippocampal function.
The preparation supports investigation of cellular mechanisms associated with neurological disease and examination of how drugs affect hippocampal function. Researchers can combine the maintained tissue with electrophysiology, imaging, or pharmacological experiments to observe changes in electrical activity, synaptic transmission, and plasticity under controlled laboratory conditions.