Journal
/
Neuroscience
/
Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology

Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology

DOI:
10.3791/2330-v

•

14:57 min

•

March 23, 2011

•

, ,
1Graduate Center for Gerontology,University of Kentucky College of Public Health, 2Department of Molecular and Biomedical Pharmacology,University of Kentucky College of Medicine, 3Sanders-Brown Center on Aging,University of Kentucky College of Medicine

Chapters

  • 00:05Title
  • 01:52Brain Removal and Hippocampal Dissection in Aged Rats
  • 06:41Brain Sectioning Using A Vibrating Microtome
  • 08:12Elicit and Record CA3-CA1 Synaptic Responses
  • 11:32Representative Results
  • 14:31Conclusion

Summary

Automatic Translation

Automatic Translation

This article outlines procedures for preparing hippocampal slices from rats and transgenic mice for the study of synaptic alterations associated with brain aging and age-related neurodegenerative diseases, such as Alzheimer’s disease.

Tags

Acute Hippocampal SlicesRatsTransgenic MiceSynaptic AlterationsAgingAmyloid PathologySynaptic FunctionPlasticityElectrophysiological TechniquesCognitive ImpairmentsSynaptic Plasticity MechanismsLong-term PotentiationLong-term DepressionNootropic CompoundsBrain AgingAlzheimer’s Disease (AD)Aged RatsAD Model Mice

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Preparation of Oligomeric β-amyloid1-42 and Induction of Synaptic Plasticity Impairment on Hippocampal Slices

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals

Preparation of Acute Subventricular Zone Slices for Calcium Imaging

Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices

Stereotaxic Infusion of Oligomeric Amyloid-beta into the Mouse Hippocampus

Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents

Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method

Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System

Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice

Preparation of Acute Human Hippocampal Slices for Electrophysiological Recordings

Read Article