Neonatal Mouse Pups
Neonatal Mouse Pups - Related Videos
09:35
Neurodevelopmental Reflex Testing in Neonatal Rat Pups
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Cited by 77 •
2017
Behavioral testing is the gold standard for determining outcomes following brain injury, and can identify the presence of developmental disabilities in infants and children. Neurodevelopmental reflexes are an early indicator of these abnormalities. A host of easily accomplished developmental reflex tests in the neonatal rodent were developed and described here.
07:18
Oral Gavage in Neonatal Mouse Pups and Functional Assessment of Gut Barrier Integrity Using Ussing Chambers
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2026
This article describes: (1) oral gavage delivery of test molecules to neonatal mice as early as DOL 6; and (2) ex vivo evaluation of colonic epithelial barrier function in biopsies from DOL 10 to weaning (DOL 20) pups by assessing paracellular and transcellular permeability with Ussing chambers.
02:53
Neonatal Mouse Ovary Isolation: A Surgical Procedure to Harvest Pair of Ovaries from Neonatal Mouse Model
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2025
In this video, we describe a surgical procedure to isolate ovaries from a neonatal mouse.
13:31
A Neonatal Mouse Spinal Cord Compression Injury Model
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Cited by 4 •
2016
This article describes a method for generating a reproducible spinal cord compression injury (SCI) in the neonatal mouse. The model provides an advantageous platform for studying mechanisms of adaptive plasticity that underlie spontaneous functional recovery.
03:26
Transplantation of Interneuron Precursor Cells into the Brain of a Mouse Pup
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2025
Source: Quattrocolo, G., et al. Homochronic Transplantation of Interneuron Precursors into Early Postnatal Mouse Brains. J. Vis. Exp. (2018)This video demonstrates the transplantation of interneuron precursor cells into the neonatal mouse brain using either a homotopic or heterotopic approach. The procedure involves securing an anesthetized recipient pup, identifying the target brain region, and injecting a suspension of precursor cells derived from a donor pup at the same developmental stage.
FAQs
These processes develop together to refine immature neural circuits. Changing neuronal activity can influence which synaptic connections are strengthened, while myelination progressively supports faster and more coordinated signaling. Studying them during the same early-life window helps researchers connect cellular maturation with emerging nervous-system function and later behavioral outcomes.
Small differences in developmental age can correspond to meaningful changes in neural structure, function, sensory experience, and behavior. Recording the age of each pup allows researchers to relate observed phenotypes to a defined stage of nervous-system maturation. This improves comparisons among experimental groups and helps distinguish developmental effects from treatment-related effects.
Maternal care and sensory input provide environmental factors that can shape developing neural circuits alongside intrinsic cellular programs. Changes in these influences may affect neuronal activity, synapse formation, and emerging behavior. Neonatal mouse pups therefore allow researchers to examine how early experiences interact with brain maturation rather than studying development as an isolated biological process.
Studies can assess cellular, structural, functional, and behavioral outcomes within the same developmental context. Relevant measures may include neuronal activity, synapse formation, myelination, neural-circuit maturation, and early-life behavioral phenotypes. Considering several outcome levels helps researchers connect changes in cells and networks with observable effects on nervous-system development.
Researchers use this early-life model to examine how cellular or environmental factors alter the developing nervous system before maturation is complete. Findings can link changes in neuronal activity, synaptic development, myelination, or behavior with disorder-related phenotypes. The approach is particularly useful for studying when developmental differences emerge and how they relate to circuit formation.
Their early developmental stage provides a context for examining how an immature nervous system responds to injury and how neural structures or functions may change afterward. Researchers can investigate repair-related outcomes alongside ongoing circuit maturation, rather than treating injury as separate from development. Defined developmental age also supports controlled comparisons across experimental conditions.