Semi Thin Microtome
Semi Thin Microtome - Related Videos
12:10
Semi-automated Optical Heartbeat Analysis of Small Hearts
0 Views •
Cited by 108 •
2009
We have developed a Semi-automated Optical Heartbeat Analysis method (SOHA) for analyzing high speed optical recordings from Drosophila, zebrafish and embryonic mouse hearts. We demonstrate the application of our methodology to the analysis of heart function in fruit fly and embryonic mouse hearts.
11:17
A Semi-quantitative Approach to Assess Biofilm Formation Using Wrinkled Colony Development
0 Views •
Cited by 32 •
2012
We provide a simple, semi-quantitative method to investigate biofilm formation in vitro. This method takes advantage of the Zeiss stemi 2000-C Dissecting Microscope (with camera attachment) to monitor both the timing and pattern of biofilm formation, as assessed by the development of wrinkled colonies.
11:52
An Isolated Semi-intact Preparation of the Mouse Vestibular Sensory Epithelium for Electrophysiology and High-resolution Two-photon Microscopy
0 Views •
Cited by 5 •
2013
Analysis of vestibular hair cell function is complicated by their location deep within the hardest part of the skull, the petrous temporal bone. Most functional hair cell studies have used acutely isolated hair cells. Here we describe a semi-intact preparation of mouse vestibular epithelium for electrophysiological and two-photon microscopy studies.
10:03
Screening for Amyloid Aggregation by Semi-Denaturing Detergent-Agarose Gel Electrophoresis
0 Views •
Cited by 185 •
2008
SDD-AGE is a useful technique for the detection and characterization of amyloid-like polymers in cells. Here we demonstrate an adaptation that makes this technique amenable to large-scale applications.
08:17
A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood
0 Views •
Cited by 9 •
2016
Whole blood Immunophenotyping is indispensable for monitoring the human immune system. However, the number of reagents required and the instability of specific fluorochromes in premixed cocktails necessitates daily reagent preparation. Here we show that semi-automated preparation of staining antibody cocktail helps establish reliable immunophenotyping by minimizing variability in reagent dispensing.
FAQs
Resin embedding follows fixation and dehydration and creates a stable block that can advance in controlled increments against a knife. This preparation supports thin sections that preserve cellular organization, allowing light microscopy to reveal neuronal layers, myelin, axonal pathology, and lesion boundaries. Those observations can guide later selection of regions for transmission electron microscopy.
The block advances in precisely controlled increments against either a glass or diamond knife, producing sections suitable for microscopic examination. This mechanical arrangement connects the instrument’s movement with the preparation of thin, organized tissue sections. In neuroscience, that consistency helps researchers inspect brain and peripheral nerve architecture before choosing regions for more detailed ultrastructural analysis.
Semi-thin sectioning provides an intermediate level of structural information. Light microscopy of resin-embedded sections can show overall organization, neuronal layers, myelin, axonal pathology, and lesion boundaries, while the same assessment helps identify promising regions for transmission electron microscopy. It therefore supports informed region selection without relying on ultrastructural analysis alone.
Neural tissue is first fixed, then dehydrated and embedded in epoxy or acrylic resin. The hardened resin block is placed for sectioning, and the block advances in controlled increments against a glass or diamond knife. The resulting sections are examined by light microscopy to assess preserved organization and identify relevant regions.
The workflow depends on fixed neural tissue, dehydration and an embedding medium such as epoxy or acrylic resin. Sectioning requires the microtome and a glass or diamond knife, while light microscopy is used to examine the sections. Together, these materials and instruments support structural assessment of brain and peripheral nerve samples.
In neuroscience, these sections can reveal neuronal layers, myelin, axonal pathology, and the boundaries of lesions in brain or peripheral nerve samples. Because the sections preserve cellular organization for light microscopy, researchers can evaluate tissue structure and use that information to select specific regions for subsequent transmission electron microscopy.