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定量组织样本中的γH2AX灶
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定量组织样本中的γH2AX灶

Article DOI: 10.3791/2063-v • 08:48 min • June 28th, 2010
June 28th, 2010 •
1,2, 1,3, 1,2,3, 1, 2,3, 3,4,5, 1,3
1Epigenomic Medicine, Baker IDI Heart and Diabetes Institute, The Alfred Medical Research and Education Precinct, 2Epigenetics in Human Health and Disease, Baker IDI Heart and Diabetes Institute, The Alfred Medical Research and Education Precinct, 3Department of Pathology, The University of Melbourne, 4Department of Allergy and Immunology, Murdoch Children's Research Institute, Royal Children's Hospital, 5Department of Pediatrics, The University of Melbourne

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定量DNA双链上的γH2AX灶的基础上休息，已经成为一个宝贵的工具，尤其是在放射生物学，组织的放射敏感性和辐射修改化合物的影响的评价。在这里，我们表现出的组织样本中γH2AX灶的定量免疫荧光法的使用。

Tags

细胞生物学，40期，免疫荧光，DNA双链断裂，组蛋白变体，H2AX的DNA损伤，电离辐射，活性氧
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