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JoVE Journal Developmental Biology
Generera 3D-sfärer och 2D-luft-vätskegränssnittskulturer av humaninducerade pluripotenta stamcellsderiverade typ 2 alveolära epitelceller
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Generera 3D-sfärer och 2D-luft-vätskegränssnittskulturer av humaninducerade pluripotenta stamcellsderiverade typ 2 alveolära epitelceller

Article DOI: 10.3791/63875-v • 07:02 min • April 15th, 2022
April 15th, 2022 •
*1,2,3, *1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, *1,2, *1,2
1Center for Regenerative Medicine, Boston University and Boston Medical Center, 2The Pulmonary Center and Department of Medicine, Boston University School of Medicine, 3QIMR Berghofer Medical Research Institute
* These authors contributed equally

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Det nuvarande protokollet beskriver humant inducerade pluripotenta stamcellsderiverade typ 2 alveolära epitelliknande celler (iAT2s). Dessa celler kan odlas som självförnyande sfärer i 3D-odling eller anpassas till luft-vätskegränssnitt (ALI) -kultur.

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Utvecklingsbiologi nummer 182
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