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IDG-SW3 3차원 세포외 기질에서의 세포 배양
JoVE 杂志
生物工程
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JoVE 杂志 生物工程
IDG-SW3 Cell Culture in a Three-Dimensional Extracellular Matrix
DOI:

10:48 min

November 13, 2023

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Chapters

  • 00:05Introduction
  • 00:33Preparation of the Cell-Matrix Mixture
  • 02:07Plating the Cell-Gel Mixture
  • 02:58Identifying the Cell Viability and Cellular Dendrites Using a Confocal Microscope
  • 06:00Identifying Appearance Using a Stereomicroscope
  • 06:55Identifying Mineral Deposition by XRF Assay
  • 07:45Identifying Functional Gene Expression
  • 08:25Results: Dendrite Network, Mineral Deposition, and Gene Expression of Osteogenic Cultured IDG-SW3 Cells
  • 10:04Conclusion

Summary

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여기에서는 3차원(3D) 세포외 기질에서 IDG-SW3 세포를 배양하기 위한 프로토콜을 제시합니다.

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