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测量体分化的离小鼠脂肪组织和原代前脂肪细胞的脂肪分解率
JoVE 杂志
生物学
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JoVE 杂志 生物学
Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro
DOI:

09:41 min

March 17, 2023

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Chapters

  • 00:04Introduction
  • 01:00Preparation of Buffers and Collection Plates
  • 01:36Preparation of Primary Preadipocyte Samples
  • 02:15Preparation of Ex Vivo Culture Samples
  • 03:44Lipolysis Assay
  • 04:33Free Fatty Acid (FFA) Colorimetric Assay
  • 05:46Glycerol Colorimetric Assay
  • 06:34Calculation of Lipolytic Rate
  • 07:18Results: Basal and Stimulated Lipolytic Rates of In Vitro Differentiated Preadipocytes and Ex Vivo Adipose Tissue
  • 09:09Conclusion

Summary

自动翻译

脂肪细胞中的甘油三酯脂肪分解是导致游离脂肪酸和甘油释放的重要代谢过程。在这里,我们提供了一个详细的方案来测量小鼠脂肪细胞和 离体 脂肪组织中的基础和刺激脂肪分解。

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