Development and Optimization of A Human Hepatocellular Carcinoma Patient-Derived Organoid Model for Potential Target Identification and Drug Discovery

Cheng-Yang Zhang; Xiao-Feng Zhang; Jun Yuan; Yuan-Feng Gong; Hui Tang; Wan-Yu Guo; Tong-Yan Li; Cai-Wen Li; Yun-Qiang Tang; Ning-Fang Ma; Ming Liu

doi:10.3791/65785

JoVE Journal
Cancer Research

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Cancer Research

Development and Optimization of A Human Hepatocellular Carcinoma Patient-Derived Organoid Model for Potential Target Identification and Drug Discovery

开发和优化用于潜在靶点识别和药物发现的人肝细胞癌患者来源类器官模型

Full Text

2,483 Views

07:25 min

August 18, 2023

DOI: 10.3791/65785-v

Cheng-Yang Zhang*^1,2, Xiao-Feng Zhang*³, Jun Yuan*^1,2, Yuan-Feng Gong⁴, Hui Tang⁴, Wan-Yu Guo^1,2, Tong-Yan Li^1,2, Cai-Wen Li^1,2, Yun-Qiang Tang⁴, Ning-Fang Ma^1,2, Ming Liu^1,2

¹Affiliated Cancer Hospital and Institute of Guangzhou Medical University, ²Guangzhou Municipal and Guangdong Provincial Key Laboratory of Protein Modification and Degradation, Center for Cancer Research and Translational Medicine, School of Basic Medical Sciences,Guangzhou Medical University, ³Department of Pediatric Surgery, Guangzhou Institute of Pediatrics, Guangzhou Women and Children’s Medical Center,Guangzhou Medical University, ⁴Department of Hepatobiliary Surgery, Affiliated Cancer Hospital,Guangzhou Medical University

Please note that some of the translations on this page are AI generated. Click here for the English version.

我们对肝细胞癌（HCC）类器官形成的现有方案进行了全面概述和改进，涵盖了类器官培养的所有阶段。该系统可作为识别潜在治疗靶点和评估候选药物有效性的宝贵模型。

我们的实验室旨在研究肝癌的分子发病机制，并确定药物开发的新治疗靶点。我们想利用新的疾病模型（如患者来源的类器官）来回答癌症如何启动、转移和进展为药物耐受性。目前的实验挑战主要体现在两个方面。

首先，缺乏一套完整的肝细胞癌类器官构建方案。其次，培养的HCC类器官的尺寸太小，无法进行后续的实验处理。在该协议中，我们提供了优化的类器官培养和随后的转移和冷冻程序的详细信息，以及在所有程序中应采取的预防措施。

我们优化了类器官培养基的组成，使其具有接近原始组织特征的类器官。我们可以使用患者来源的类器官监测肿瘤的演变，并尝试确定用于HCC治疗的新分子和治疗靶点。

View the full transcript and gain access to thousands of scientific videos

Explore More Videos

癌症研究第198期靶点鉴定药物发现发病机制治疗天然肿瘤组织体外培养肝癌培养方案 HCC类器官传代冷冻保存复苏遗传相似性治疗靶点药物开发