Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove

32.17: Peptide Identification Using Tandem Mass Spectrometry

JoVE Core
Cell Biology

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

Peptide Identification Using Tandem Mass Spectrometry

32.17: Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.

This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of proteomics.

The different peptides obtained after enzymatic digestion can be further separated as much as possible as per the physical size and/or chemical properties using sophisticated instruments like gel electrophoresis or liquid chromatography.

This first stage of MS/MS allows the peptides to separate based on their mass-to-charge ratio, followed by breaking or fragmenting the selected peptide ion in the collision cell. The second mass analyzer helps build the fragmentation pattern to determine the sequence or identify the protein.

Studying the results and deducing the peptide sequence is extremely important once the spectrum is obtained. For this, numerous protein database search algorithms and bioinformatics tools help in sample elucidation from the obtained spectrum. In the case of an unknown protein, the obtained spectrum shows numerous overlapping fragments. However, as the spectral pattern is unique for a given protein, the analysis software compares the obtained spectrum with a database of known peptide sequences, thus elucidating the unknown protein from the overlapping fragments.

Different analyzer combinations can be used to create hybrid MS/MS instruments and thus increase the sensitivity of the results. For example, quadrupole time-of-flight (QTOF) is a combination of quadrupole and time-of-flight mass analyzers. The triple quadrupole mass spectrometer contains two quadrupole mass analyzers separated by a non-mass resolving quadrupole collision cell for fragmentation.

In newborn screening, the tandem MS uses dried blood-spot samples to help in a comprehensive assessment of inborn metabolic disorders like phenylketonuria, sickle cell disease, etc. The early identification helps substantial improvement in health outcomes.

Suggested Reading


Keywords: Peptide Identification Tandem Mass Spectrometry MS/MS MS2 Protein Identification Proteomics Enzymatic Digestion Gel Electrophoresis Liquid Chromatography Mass-to-charge Ratio Fragmentation Pattern Protein Database Search Bioinformatics Tools QTOF Triple Quadrupole Newborn Screening Inborn Metabolic Disorders

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter