Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove

32.18: X-ray Diffraction of Biological Samples

TABLE OF
CONTENTS
JoVE Core
Cell Biology

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

Education
X-ray Diffraction of Biological Samples
 
TRANSCRIPT

32.18: X-ray Diffraction of Biological Samples

X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.

According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal crystal planes. The scattering angle of the rays in specific directions by the atoms within the sample provides a diffraction pattern representing the electron density due to the atoms and bonds within the crystal. This information about the crystal lattice arrangement helps identify the material from its diffraction pattern, which resembles  a fingerprint. The material can be identified by comparing this fingerprint with a database, such as  the International Center for Diffraction Database (ICDD). XRD can look at the size, shape, and internal structure and help in the basic structural determination of a sample or unknown material.

For a single crystal, a typical diffraction pattern comprises spots that are 2D slices of 3-dimensional spheres. A computer program can integrate the resulting spots to determine the shape and intensity of the diffracted X-rays. Whereas, in a powder sample, the X-rays interact with many tiny crystals in random orientations. Hence, instead of spots, a circular diffraction pattern is observed. The intensities of the diffracted circles are plotted against the angles between the ring of the beam axis, giving a 2D plot known as a powder pattern. Amorphous or non-crystalline powders give a broad peak due to scattering from different directions compared to ordered scattering in crystalline powders, giving sharp concentric rings.

Certain precautions should be taken while studying biological samples using this technique. First of all, well-ordered crystals are a prerequisite for deciphering the protein structure through single-crystal XRD. Generally, single small crystals with well-defined faces, if they contain heavier atoms, will suffice and give a good diffraction pattern. For organic compounds, however, the crystals need to be larger. Without well-defined, viable crystals, this technique is not feasible. Some molecules are inherently more crystalline than others; thus, the difficulty of obtaining X-ray quality crystals can vary between compounds and is the main limiting factor of this technique. Further, the powder samples should be fine powder and have no clumps.


Suggested Reading

Tags

Keywords: X-ray Diffraction XRD Bragg's Law Crystalline Structures Electron Density Crystal Lattice Powder Diffraction Single Crystal Amorphous Samples Biological Samples Protein Structure

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter