Xrf Nano-probe Imaging
Xrf Nano-probe Imaging - Related Videos
07:45
X-ray Fluorescence (XRF)
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2023
Source: Laboratory of Dr. Lydia Finney — Argonne National Laboratory X-ray fluorescence is an induced, emitted radiation that can be used to generate spectroscopic information. X-ray fluorescence microscopy is a non-destructive imaging technique that uses the induced fluorescence emission of metals to identify and quantify their spatial distribution.
07:28
Measurement of Bioelectric Current with a Vibrating Probe
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Cited by 15 •
2011
The manufacture, calibration and use of non-invasive vibrating probes to measure bioelectric current in various biological systems is described.
10:27
Fabrication of Nano-engineered Transparent Conducting Oxides by Pulsed Laser Deposition
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Cited by 15 •
2013
We describe the experimental method to deposit nanostructured oxide thin films by nanosecond Pulsed Laser Deposition (PLD) in the presence of a background gas. By using this method Al-doped ZnO (AZO) films, from compact to hierarchically structured as nano-tree forests, can be deposited.
06:52
Nanocrystalline Alloys and Nano-grain Size Stability
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2023
Source: Sina Shahbazmohamadi and Peiman Shahbeigi-Roodposhti-Roodposhti, School of Engineering, University of Connecticut, Storrs, CT Alloys with grain size less than 100 nm are known as nanocrystaline alloys. Due to their enhanced physical and mechanical properties, there is an ever-increasing demand to employ them in various industries such as semiconductor, biosensors and aerospace. To improve the processing and application of nanocrystalline alloys, it is necessary to develop close to 100%...
13:58
Probing C84-embedded Si Substrate Using Scanning Probe Microscopy and Molecular Dynamics
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Cited by 1 •
2016
This paper reports the nanomaterial fabrication of a fullerene Si substrate inspected and verified by nanomeasurements and molecular dynamic simulation.
FAQs
The emitted fluorescence carries two kinds of information: its characteristic X-ray energies indicate which elements produced the signal, while the measured signal abundance indicates their local concentrations. This separation allows researchers to distinguish elemental species within a biochemical sample and compare where those elements are more or less concentrated across the imaged region.
The focused X-ray beam confines atomic excitation to a very small region of the sample. As the resulting fluorescence is associated with that localized interaction, measurements can preserve information about where each element occurs at nanometer-scale resolution. This spatial precision is essential for connecting elemental distributions with cellular, tissue, protein, or biomaterial structure.
Because the method does not require fluorescent labels, elemental information can be obtained directly from the sample's characteristic X-ray emissions. This enables researchers to investigate metals and other elements as chemical components of biological systems rather than relying on an added fluorescent marker. The approach is therefore useful for examining elemental composition in biochemical contexts.
Elemental maps do more than show whether an element is present. Their spatial patterns reveal how elemental organization corresponds to biological structure and activity, while local abundance measurements add concentration information. In biochemistry, this relationship helps researchers examine how the distribution of metals or other elements may relate to cellular processes, proteins, tissues, or biomaterials.
A basic workflow examines a sample with a focused X-ray beam, allowing atoms in the illuminated region to emit characteristic fluorescent X-rays. Those emissions are analyzed to identify the elements present and estimate their local concentrations. Repeating the measurement across the sample produces chemical maps that show the spatial distribution of the detected elements.
XRF nano-probe imaging can be applied to cells, tissues, proteins, and biomaterials, while detecting metals and other elements. This breadth lets investigators connect elemental distributions to different levels of biological organization, from cellular or tissue patterns to the composition of isolated biochemical materials. It therefore supports both biological and biomaterial-focused investigations.
In biochemical research, XRF nano-probe imaging can address metal homeostasis, enzyme function, and nutrient transport. Its maps show where relevant elements are located and how abundant they are locally, helping relate elemental organization to biochemical roles. The measurements can also support comparisons among biological regions when elemental localization is central to the research question.
Disease-related accumulation is another use case. By locating and estimating the abundance of elements in cells or tissues, the technique can reveal spatial patterns associated with elemental buildup. Researchers can then relate those patterns to biological structure and activity, providing a basis for investigating how elemental distributions correspond to disease-related biochemical changes.