Mouse Lung Epithelial Cells
Mouse Lung Epithelial Cells - Related Videos
12:31
Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds
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Cited by 8 •
2016
This protocol efficiently directs mouse embryonic stem cell-derived definitive endoderm to mature airway epithelial cells. This differentiation technique uses 3-dimensional decellularized lung scaffolds to direct lung lineage specification, in a defined, serum-free culture setting.
03:15
Analyzing the Effects of Cigarette Smoke on Antimicrobial Defense in Lung Epithelial Cells
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2026
Source: Li, T., et al. Studying Effects of Cigarette Smoke on Pseudomonas Infection in Lung Epithelial Cells. J. Vis. Exp. (2020).This video demonstrates how cigarette smoke extract weakens antimicrobial defense in lung epithelial cells by reducing AMP production, leading to increased bacterial invasion. The procedure measures intracellular bacterial load to assess the impact of smoke exposure on host-pathogen interactions.
06:02
Isolation of Live Myeloid and Epithelial Cell Populations from the Mouse Lung
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Cited by 2 •
2025
This protocol details the isolation of live immune and non-immune populations from the mouse lung at a steady state and following influenza infection. It also provides gating strategies for identifying epithelial and myeloid cell subsets.
06:14
Isolation of Alveolar Type II Epithelial Cells from Neonatal, Juvenile, and Adult Murine Lungs Adaptable to Infectious Experimental Settings
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2025
This protocol presents a flow cytometry-based method to isolate viable alveolar type II epithelial cells (AECII) from neonatal, juvenile, or adult murine lungs (infected or non-infected). This approach enables downstream molecular and/or functional studies of AECII to identify their roles during lung inflammation and infection.
09:04
Isolation of Mouse Lung Dendritic Cells
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Cited by 17 •
2011
A highly purified preparation of mouse lung dendritic cells is described. Specific emphasis is given to the isolation of conventional dendritic cell subset.
FAQs
Polarized layers organize mouse lung epithelial cells into distinct apical and basal surfaces, allowing the culture to reproduce directional features of respiratory tissue. Cell-cell junctions connect neighboring cells and contribute to barrier function, while the resulting architecture supports studies of how epithelial integrity changes during repair or under engineered conditions.
Extracellular matrix signals help regulate barrier function, repair, and differentiation rather than serving only as structural support. Changing these signals can alter how the cells organize and mature, making matrix conditions an important variable in bioengineered lung models. Researchers can therefore examine how the surrounding environment influences epithelial behavior.
Engineered environments can modify the signals that mouse lung epithelial cells receive, affecting their organization, barrier properties, repair responses, and differentiation. This makes them useful for testing how designed tissue settings influence respiratory cell behavior. In bioengineering, such models help connect environmental design choices with the biological performance of an epithelial layer.
A basic workflow begins by culturing the cells under conditions that allow them to establish a polarized layer with apical and basal surfaces. Researchers then examine features relevant to their question, such as cell-cell junctions, barrier function, repair, or differentiation. The resulting culture can serve as a controlled platform for studying engineered lung environments.
Researchers use these cells when they need to determine how a biomaterial affects respiratory epithelial behavior. The culture can reveal whether the engineered environment supports organized layers, barrier function, repair, or differentiation. This information helps compare material designs before applying them in more complex in vitro lung models or therapeutic-testing platforms.
These models can support investigations of tissue injury and regeneration, disease-related lung biology, biomaterial performance, and therapeutic testing. Their value comes from linking epithelial organization and function with controllable engineered conditions. Researchers can use the resulting observations to assess how closely a constructed platform reproduces relevant aspects of respiratory tissue behavior.