Organ Mounting
Organ Mounting - Related Videos
09:16
Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts
0 Views •
Cited by 4 •
2017
We present a method for isolation and culture of the mouse Wolffian duct (WD). We also demonstrate a detailed procedure for whole mount immunostaining of cultured/freshly isolated WDs with fluorescently tagged antibodies. Together, these techniques enable the study of WD development, coiling, and differentiation.
09:17
Whole-mount Clearing and Staining of Arabidopsis Flower Organs and Siliques
0 Views •
Cited by 13 •
2018
In this protocol, we describe techniques for the proper dissection of Arabidopsis flowers and siliques, some basic clearing techniques, and selected staining procedures for whole-mount observations of reproductive structures.
08:42
Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos
0 Views •
Cited by 3 •
2016
Techniques are described to immunostain phospho-epitopes in whole zebrafish embryos and then conduct two-color fluorescent confocal localization in cellular structures as small as primary cilia. The techniques for fixing and imaging can define the location and kinetics of the appearance or activation of specific proteins.
03:43
A Whole-Mount Organ Staining Technique for Three-Dimensional Imaging of the Kidney
0 Views •
2025
This video demonstrates an assay for the whole-mount staining of murine kidneys using a tissue-clearing method called CUBIC (clear, unobstructed brain/body imaging cocktails and computational analysis) and immunofluorescent staining. The kidney is subjected to tissue clearing, wherein the kidney is delipidated, decolorized, and treated with a refractive index matching solution to make the tissue transparent for optimum three-dimensional imaging. The tissue is stained with fluorescently labeled...
08:00
Whole-Mount In Situ Hybridization
0 Views •
2023
Whole-mount in situ hybridization (WMISH) is a common technique used for visualizing the location of expressed RNAs in embryos. In this process, synthetically produced RNA probes are first complementarily bound, or "hybridized," to the transcripts of target genes. Immunohistochemistry or fluorescence is then used to detect these RNA hybrids, revealing spatial and temporal patterns of gene expression. Unlike traditional in situ hybridization techniques, which require thin tissue sections whose...
FAQs
A suitable mounting arrangement restrains unwanted movement without covering the surfaces needed for examination, imaging, testing, or manipulation. Orientation therefore becomes a functional decision rather than a purely mechanical one. If the support blocks relevant structures or limits access, it can reduce the value of otherwise detailed measurements and make relationships between tissue structure and function harder to evaluate.
Some examinations require the specimen to remain hydrated or within physiological conditions while it is positioned. Preserving these conditions helps the mounted tissue remain appropriate for the intended observation or test, especially in perfusion studies and bioengineered systems. The mounting environment must therefore provide stability while avoiding changes that could compromise tissue characterization or interpretation.
Orientation determines which structures are visible, how surfaces relate to the imaging field, and how consistently measurements can be repeated. A poorly oriented specimen may obscure relevant anatomy or introduce variation between observations. Careful positioning improves image quality and measurement consistency, helping researchers connect three-dimensional tissue organization with biomechanical behavior or other functional results.
Reproducibility depends on consistently positioning the specimen, limiting movement, and preserving the conditions required by the examination. The support, chamber, or device should provide a repeatable relationship between the organ and the measurement or imaging system. This consistency reduces positioning-related variation, allowing results from organ biomechanics, microscopy, or tissue characterization studies to be compared more reliably.
Preparation begins by determining the structures, surfaces, and measurements that must remain accessible. The organ or section is then oriented within an appropriate support, chamber, or device and secured to limit movement. When required, hydration or physiological conditions are maintained throughout the examination. The final arrangement should preserve access while supporting consistent imaging, testing, or manipulation.
Researchers use this approach when an intact organ or organ section must remain positioned during imaging, testing, or engineered manipulation. In bioengineering, it supports organ biomechanics, tissue characterization, perfusion studies, microscopy, and organ-on-a-chip research. Its value is greatest when reliable positioning is needed to examine complex three-dimensional tissue and relate structural features to function.
A well-mounted specimen can support observations that depend on stable positioning and, when necessary, maintained physiological conditions. In perfusion studies and organ-on-a-chip research, this stability helps preserve access for examining tissue behavior within an engineered setup. The resulting observations can contribute to analyses of tissue structure, function, characterization, and responses during testing.