Whole-mount Clearing and Staining of Arabidopsis Flower Organs and Siliques

This protocol describes how to properly dissect flower buds, flowers and young siliques. And the subsequent whole-mounting clearing for diverse staining and observation techniques. This method can help answer key questions in the sexual plant production field.

Such as a steadily mutant analysis or any environmentally and experimentally induced failure in flower development and function. The main advantage of this technique is to allow for easy and quick screening of many developmental stages simultaneously. Visual demonstration of this method is critical, as flower organ dissection steps are difficult to learn because they require prior knowledge of flower anatomy and dissection skills.