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Whole-mount Clearing and Staining of Arabidopsis Flower Organs and Siliques
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Developmental Biology
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JoVE Journal Developmental Biology
Whole-mount Clearing and Staining of Arabidopsis Flower Organs and Siliques

Whole-mount Clearing and Staining of Arabidopsis Flower Organs and Siliques

DOI:
10.3791/56441-v

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09:17 min

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April 12, 2018

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, , ,
1Department of Plant and Microbial Biology, Zurich-Basel Plant Science Center,University of Zurich

Chapters

  • 00:04Title
  • 00:56Flower and Silique Fixation and Dissection Under the Stereomicroscope
  • 02:05Chloral Hydrate-based Clearing and Combined Clearing-staining
  • 03:28Combined Alexander Staining and Herr’s 4½ Clearing of Anthers
  • 04:29Removing the Exine from Pollen Grains
  • 05:49Sodium Dodecyl Sulfate (SDS) Clearing
  • 07:04Results: Arabidopsis Flowers and Floral Organs are Dissected, Cleared, and Stained for Whole Mount Observations of Reproductive Structures
  • 08:41Conclusion

Summary

Automatic Translation

Automatic Translation

In this protocol, we describe techniques for the proper dissection of Arabidopsis flowers and siliques, some basic clearing techniques, and selected staining procedures for whole-mount observations of reproductive structures.

Tags

Keywords: ArabidopsisFlower OrgansSiliquesWhole-mount ClearingStainingDissectionFlower DevelopmentSexual Plant ReproductionMicroscopyClearing Solution

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