Abdominal Ganglion Dissection
Abdominal Ganglion Dissection - Related Videos
07:50
Location, Dissection, and Analysis of the Murine Stellate Ganglion
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Cited by 15 •
2020
Pathophysiological changes in the cardiac autonomic nervous system, especially in its sympathetic branch, contribute to the onset and maintenance of ventricular arrhythmias. In the present protocol, we show how to characterize murine stellate ganglia to improve the understanding of the underlying molecular and cellular processes.
11:08
Dissection and Culture of Chick Statoacoustic Ganglion and Spinal Cord Explants in Collagen Gels for Neurite Outgrowth Assays
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Cited by 9 •
2011
We demonstrate how to dissect and culture chick E4 statoacoustic ganglion and E6 spinal cord explants. Explants are cultured under serum-free conditions in 3D collagen gels for 24 hours. Neurite responsiveness is tested with growth factor-supplemented medium and with protein-coated beads.
13:32
Abdominal Exam IV: Acute Abdominal Pain Assessment
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2023
Source: Joseph Donroe, MD, Internal Medicine and Pediatrics, Yale School of Medicine, New Haven, CT Abdominal pain is a frequent presenting concern in both the emergency department and the office setting. Acute abdominal pain is defined as pain lasting less than seven days, while an acute abdomen refers to the abrupt onset of severe abdominal pain with features suggesting a surgically intervenable process. The differential diagnosis of acute abdominal pain is broad; thus, clinicians must have a...
06:50
Abdominal Exam II: Percussion
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2023
Source: Alexander Goldfarb, MD, Assistant Professor of Medicine, Beth Israel Deaconess Medical Center, MA Medical percussion is based on the difference in pitch between the sounds elicited by tapping on the body wall. The auditory response to tapping depends on the ease with which the body wall vibrates, and is influenced by underlying organs, strength of the stroke, and the state of the body wall. There are three main medical percussion sounds: resonance (heard over lungs), tympany (heard over...
04:41
Isolating Vestibular Ganglion Neurons From a Mouse Inner Ear
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2025
This video showcases a method for isolating vestibular neurons from a mouse pup's inner ear. It meticulously outlines the steps involved in dissecting the inner ear, harvesting the vestibular ganglion, and subsequently dissociating the ganglion into single cells. These cells are then cultured on glass-bottom dishes to establish a vestibular neuron culture.
FAQs
Preserving these connections maintains the anatomical relationships needed to examine neural organization and circuit structure. An intact preparation can help researchers relate the ganglion to incoming or outgoing nerve pathways rather than viewing isolated tissue without context. This is especially useful when studying how neural circuits contribute to motor control or other aspects of nervous-system function.
Microscopy can reveal neuronal morphology and the arrangement of structures within the ganglion, while physiological recording can provide functional information about neural activity and signaling. Examining both forms of evidence allows researchers to compare nervous-system structure with function. Together, these approaches support investigation of neural circuits, synaptic signaling, and the organization of an accessible invertebrate nervous system.
The preparation provides a controlled way to examine neural circuits and motor-control pathways that may contribute to behavior. Researchers can analyze the physical organization of neurons and nerves alongside functional observations from the preparation. This structural and functional comparison helps clarify how patterns of nervous-system organization relate to the actions produced by an invertebrate.
The procedure begins with exposing the relevant region of the invertebrate and working under magnification. Surrounding body tissues are then removed carefully so the ganglion and connected nerves remain intact. Depending on the experiment, the ganglion may be examined directly for microscopy or transferred to a preparation suitable for physiological recording. The exact handling depends on the intended analysis.
The ganglion and its nerves must remain identifiable and sufficiently intact for later anatomical or physiological analysis. Magnification helps the investigator distinguish nervous tissue from surrounding body tissues during removal. Careful dissection therefore improves the usefulness of the preparation by preserving the structures needed to study neuronal morphology, circuit organization, and neural function.
Researchers may choose it when they need a controlled preparation for examining an invertebrate nervous system at both anatomical and functional levels. It can support microscopy of neuronal structures, physiological studies of signaling, and analysis of circuits involved in motor control. Because the nervous system is relatively accessible, the technique is also useful for linking neural organization with behavior.