Genetically Tagged Cells
Genetically Tagged Cells - Related Videos
05:45
In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin
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Cited by 4 •
2019
Morphological changes occur in immune responsive fibroblast cells following activation and promote alterations in cellular recruitment. Utilizing 2-photon imaging in conjunction with a genetically engineered Fibroblast-specific protein 1 (FSP1)-cre; tdTomato floxed-stop-floxed (TB/TB) mouse line and green fluorescently tagged lipopolysaccharide-FITC, we can illustrate highly specific uptake of lipopolysaccharide in dermal fibroblasts and morphological changes in vivo.
08:50
Genetic Screens
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2023
Genetic screens are critical tools for defining gene function and understanding gene interactions. Screens typically involve mutating genes and then assessing the affected organisms for phenotypes of interest. The process can be “forward”, where mutations are generated randomly to identify unknown genes responsible for the phenotypes, or it can be “reverse”, where specific genes are targeted for mutation to observe what phenotypes are produced.Here, JoVE reviews various types of genetic...
03:17
Luciferase-tagged Glioma Stem Cell Model: A Lentivirus-mediated Method to Prepare Luciferase-tagged Glioma Stem Cells for In Vitro Therapeutic Studies
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2023
This video demonstrates the establishment of luciferase-tagged glioma stem cells via lentivirus-mediated gene delivery. Luciferase acts as a reporter gene and helps facilitate rapid drug screening experiments.
10:38
An Overview of Genetic Engineering
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2023
Genetic engineering – the process of purposefully altering an organism’s DNA – has been used to create powerful research tools and model organisms, and has also seen many agricultural applications. However, in order to engineer traits to tackle complex agricultural problems such as stress tolerance, or to realize the promise of gene therapy for treating human diseases, further advances in the field are still needed. Important considerations include the safe and efficient delivery of genetic...
10:47
An Overview of Genetics and Disease
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2023
Many human diseases are associated with mutations or variations in genetic sequences. Some of these genetic variants are heritable, passed down from generation to generation, while others arise sporadically during an organism’s life and cause diseases such as cancer. Researchers are trying to identify and characterize these genetic alterations in the hopes of improving diagnosis and therapeutic options for patients.In this video, we will examine the history of genetic disease research, and...
FAQs
The selected promoter determines when and where the reporter gene is expressed, linking the detectable marker to a particular pattern of gene activity. This makes promoter choice central to interpreting results: a marker can indicate the identity or activity of cells only in relation to the regulatory sequence controlling it. Researchers therefore use promoter-driven expression to investigate cell states and biological processes.
Fluorescent markers can reveal the location of tagged cells within living or fixed biological systems and help distinguish those cells from surrounding populations. Their signal can also provide information about cellular identity or activity when expression is linked to an appropriate promoter. Imaging supplies spatial information, whereas molecular analysis can further examine the tagged population and its expression-related properties.
Imaging shows where genetically tagged cells are located and can support observation of their distribution or movement within a biological system. Molecular analysis provides a complementary way to examine the tagged cells and their associated gene-expression information. Using either approach, or both, helps connect the cells’ physical position with their identity or activity in the study.
A typical workflow begins by selecting a reporter gene and a promoter suited to the biological question. Researchers then introduce the construct into the cells and identify cells that express the marker. The tagged population can subsequently be examined by imaging or molecular analysis in living or fixed systems, depending on whether the goal is to follow position, identity, activity, or expression.
For lineage tracing, the marker allows researchers to follow tagged cells and examine their descendants or distribution over time. In migration studies, observing the marker reveals where the cells move within a biological system. These uses make the approach valuable for investigating how cells contribute to development, physiology, or disease-related processes.
Genetically tagged cells help researchers evaluate the behavior of cells placed in engineered tissues or used therapeutically. Detecting the marker over time can show where those cells are located and support assessment of their persistence or distribution within the system. The approach also enables investigation of cell–cell interactions, which can clarify how introduced cells relate to surrounding biological environments.