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In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin
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Bioengineering
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JoVE Journal Bioengineering
In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin

In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin

DOI:
10.3791/59647-v

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05:45 min

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July 11, 2019

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, , ,
1School of Brain and Behavioral Science, Center for Advanced Pain Studies,University of Texas at Dallas

Chapters

  • 00:04Title
  • 00:55Imaging Setup
  • 02:12In Vivo Imaging
  • 04:07Results: Representative FITC-LPS Uptake
  • 05:03Conclusion

Summary

Automatic Translation

Automatic Translation

Morphological changes occur in immune responsive fibroblast cells following activation and promote alterations in cellular recruitment. Utilizing 2-photon imaging in conjunction with a genetically engineered Fibroblast-specific protein 1 (FSP1)-cre; tdTomato floxed-stop-floxed (TB/TB) mouse line and green fluorescently tagged lipopolysaccharide-FITC, we can illustrate highly specific uptake of lipopolysaccharide in dermal fibroblasts and morphological changes in vivo.

Tags

Keywords: 2-photon ImagingGenetically-tagged Reporter CellsSkinIn VivoFluorescent ProteinsMouse PawAnesthesiaStereotaxic ApparatusLaser ExcitationFocal Plane

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