Primary Treatment
Primary Treatment - Related Videos
09:21
Tumorsphere Derivation and Treatment from Primary Tumor Cells Isolated from Mouse Rhabdomyosarcomas
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Cited by 2 •
2019
This protocol describes a reproducible method for isolation of mouse rhabdomyosarcoma primary cells, tumorsphere formation and treatment, and allograft transplantation starting from tumorspheres cultures.
08:29
Ex Vivo Treatment Response of Primary Tumors and/or Associated Metastases for Preclinical and Clinical Development of Therapeutics
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Cited by 12 •
2014
Established cancer cell lines and xenografts have been the mainstay of cancer research for the past several decades. However, recent evidence suggests that therapeutic response is greatly influenced by the tumor cell microenvironment. Therefore, we have developed an ex vivo analysis of primary tumor specimens for drug development purposes.
08:43
Primary Neuronal Cultures
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2023
The complexity of the brain often requires neuroscientists to use a simpler system for experimental manipulations and observations. One powerful approach is to generate a primary culture by dissecting nervous system tissue, dissociating it into single cells, and growing those cells in vitro. Primary cultures make neurons and glia easily accessible to the experimental tools required for techniques like genetic manipulation and time-lapse imaging. Furthermore, these cultures represent a highly...
08:14
Using Primary Neurosphere Cultures to Study Primary Cilia
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Cited by 3 •
2017
The primary cilium is fundamentally important in neural progenitor cell proliferation, neuronal differentiation, and adult neuronal function. Here, we describe a method to study ciliogenesis and the trafficking of signaling proteins to cilia in neural stem/progenitor cells and differentiated neurons using primary neurosphere cultures.
11:39
The Goeckerman Regimen for the Treatment of Moderate to Severe Psoriasis
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Cited by 26 •
2013
Psoriasis is a chronic, immune-mediated inflammatory skin disease. The Goeckerman regimen, formulated for the treatment of psoriasis, consists of exposure to ultraviolet B (UVB) light and application of crude coal tar (CCT). The following protocol is for the administration of Goeckerman therapy for the treatment of moderate-to-severe psoriasis.
FAQs
Selection begins with the diagnosis and an assessment of the condition’s main biological target. Clinicians also consider disease severity and the patient’s condition when choosing an intervention. This process helps match treatment intensity and type to the underlying problem rather than relying on a single approach for every disease or injury.
The intervention may act on several kinds of targets, depending on the condition. It can remove abnormal tissue, suppress infectious agents, replace a deficient biological function, or support recovery while damaged tissues repair themselves. Identifying the relevant target determines whether treatment focuses on eliminating a cause, correcting a deficit, or enabling biological recovery.
A treatment’s mechanism influences what it can accomplish biologically. Addressing the main cause or mechanism can control disease, while correcting a deficient function may help restore physiology. In other cases, supporting repair may reduce the effects of damage without directly removing its cause. Matching mechanism to condition therefore affects the expected therapeutic outcome.
Disease severity helps determine how urgently and intensively the main problem must be addressed. The patient’s condition adds another layer of decision-making because the same biological intervention may not suit every individual. Considering both factors supports a treatment plan that is appropriate to the disorder and compatible with the patient’s circumstances.
Application begins by establishing a diagnosis and identifying the disease, injury, or disorder’s principal biological problem. The intervention is then selected according to the target, severity, and patient condition. After the initial therapy addresses that problem, clinicians may add maintenance, supportive, or rehabilitative care as needed to continue recovery or preserve function.
Its outcomes may include control of the condition, restoration of physiological function, reduction of symptoms, or support for tissue recovery. The result depends on the biological problem and the intervention selected. In clinical biology, the initial treatment also establishes a foundation for later care when ongoing maintenance, support, or rehabilitation remains necessary.