Protein Transduction
Protein Transduction - Related Videos
12:24
Mimicking the Function of Signaling Proteins: Toward Artificial Signal Transduction Therapy
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2016
We present guidelines for developing synthetic 'chemical transducers' that can induce communication between naturally unrelated proteins. In addition, detailed protocols are presented for synthesizing and testing a specific 'transducer' that enables a growth factor to activate a detoxifying enzyme and consequently, to regulate the cleavage of an anticancer prodrug.
02:19
Lentiviral-Mediated Transduction of Human Neurons for Tau Protein Aggregation Analysis
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2025
This video demonstrates the process of transducing human neuronal cells with a lentivirus encoding a mutant human tau protein tagged with yellow fluorescent protein (YFP) to study protein aggregation. It outlines the steps from viral entry to mutant tau expression, culminating in the formation of visible cytoplasmic tau aggregates in the neurons.
01:16
Transduction
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2025
Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome are...
03:13
In Utero Viral Transduction in a Mouse
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2025
Source: Lutz, D., et al. Assessment of Ultrastructural Neuroplasticity Parameters After In Utero Transduction of the Developing Mouse Brain and Spinal Cord. J. Vis. Exp. (2019).This video demonstrates viral transduction via in-utero injection in mouse embryos. A pregnant mouse is prepared for the surgery, and a midline incision is made to expose the uterine horns. A mixture of a virus containing the desired gene and a tracking dye is injected into the embryos within the uterine sacs at the...
05:28
Targeted Expression of GFP in the Hair Follicle Using Ex Vivo Viral Transduction
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Cited by 1 •
2008
The hair follicle is a highly complex appendage of the skin containing a multiplicity of cell types. The follicle undergoes constant cycling through the life of the organism including growth and resorption with growth dependent on specific stem cells. The targeting of the follicle by genes and stem cells to change its properties, in particular, the nature of the hair shaft is, discussed. Hair follicle delivery systems are described, such as liposomes and viral vectors for gene therapy. The...
FAQs
Protein transduction domains and cell-penetrating peptides act as delivery components that facilitate movement across the plasma membrane. After crossing this barrier, they support release of the attached functional protein within the cytoplasm or nucleus. The protein’s intracellular location determines which cellular processes it can influence, making delivery and release central to the method’s biological effects.
Protein Transduction changes cellular behavior by supplying a functional protein rather than introducing DNA. This distinction means the approach can produce temporary effects and avoids genomic integration, a feature that is important when researchers need experimental control without permanently altering the cell’s genetic material. It therefore complements, rather than duplicates, DNA-based strategies for cellular manipulation.
Release into the cytoplasm or nucleus places the delivered protein near the cellular machinery it may affect. In those compartments, the protein can be used to study signaling, gene regulation, or cell differentiation. The resulting location-specific activity helps researchers connect a protein’s function with changes in cellular behavior and investigate mechanisms underlying disease.
A basic workflow begins by associating the functional protein with a protein transduction domain or cell-penetrating peptide. The resulting delivery system is then applied to living cells so the protein can cross the plasma membrane and become available in the cytoplasm or nucleus. Researchers can subsequently examine changes in cellular behavior relevant to the experiment.
Researchers may choose Protein Transduction when they need to alter cellular behavior without introducing DNA or genomic integration. The approach is especially relevant to experiments examining signaling, gene regulation, cell differentiation, or disease mechanisms. Its ability to produce temporary effects makes it useful when investigators want functional protein activity without committing cells to a permanent genetic change.
In therapeutic research, Protein Transduction provides a strategy for delivering functional proteins directly to living cells. Its temporary effects and avoidance of genomic integration support interest in safer cell-based and regenerative therapies. These properties allow investigators to explore how protein delivery might influence cellular behavior while limiting reliance on approaches that permanently modify cellular genetic material.