Psd 95 Gfp
Psd 95 Gfp - Related Videos
07:59
Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System
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Cited by 2 •
2016
We established the photoconvertible PSmOrange system as a powerful, straight-forward and cost inexpensive tool for in vivo cell tracking in GFP transgenic backgrounds. This protocol describes its application in the zebrafish model system.
07:25
Live Imaging of GFP-labeled Proteins in Drosophila Oocytes
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Cited by 5 •
2013
A protocol for live imaging of GFP-tagged proteins or autofluorescent structures in individual Drosophila oocytes is described.
07:55
Imaging the Intracellular Trafficking of APP with Photoactivatable GFP
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Cited by 6 •
2015
While the transport of cell surface proteins is relatively easily studied, visualizing the trafficking of intracellular proteins is much more difficult. Here, we use constructs incorporating photoactivatable GFP and demonstrate a method to accurately follow the amyloid precursor protein from the Golgi apparatus to down-stream compartments and follow its clearance.
06:19
In Vivo Imaging of Cx3cr1gfp/gfp Reporter Mice with Spectral-domain Optical Coherence Tomography and Scanning Laser Ophthalmoscopy
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Cited by 5 •
2017
This protocol describes how high-resolution imaging techniques such as spectral domain optical coherence tomography and scanning laser ophthalmoscopy can be utilized in small rodents, using an ophthalmic imaging platform system, to obtain information on retinal thickness and microglial cell distribution, respectively.
05:28
Targeted Expression of GFP in the Hair Follicle Using Ex Vivo Viral Transduction
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Cited by 1 •
2008
The hair follicle is a highly complex appendage of the skin containing a multiplicity of cell types. The follicle undergoes constant cycling through the life of the organism including growth and resorption with growth dependent on specific stem cells. The targeting of the follicle by genes and stem cells to change its properties, in particular, the nature of the hair shaft is, discussed. Hair follicle delivery systems are described, such as liposomes and viral vectors for gene therapy. The...
FAQs
The PSD-95 component provides the targeting information that directs the fusion protein toward postsynaptic densities in neurons. As a result, the GFP signal becomes concentrated in synapse-associated puncta rather than appearing uniformly throughout the cell. This localization connects fluorescence patterns with the organization of postsynaptic structures and supports analysis of their distribution.
GFP supplies the detectable fluorescent signal needed for microscopy. Once the PSD-95 portion positions the fusion protein at postsynaptic sites, GFP makes those locations visible in living cells. Researchers can therefore link molecular localization to observed cellular structures and follow changes in synapse-associated puncta without relying only on fixed structural observations.
The puncta provide visible indicators of where the fusion protein accumulates at postsynaptic structures. Their presence and distribution can be examined to study how synapse-associated organization changes during formation, maturation, and remodeling. This makes the marker useful for connecting the spatial arrangement of a postsynaptic scaffolding protein with broader changes in neuronal connectivity.
By combining neuronal expression with fluorescence microscopy, researchers can observe synapse-associated puncta repeatedly over time. This dynamic approach allows changes in postsynaptic structures to be monitored as they occur, rather than assessed only at one endpoint. The resulting observations can reveal patterns of synaptic formation, maturation, or remodeling during cellular and developmental processes.
PSD-95-GFP supports investigations of synapse formation, maturation, remodeling, and protein organization in cellular and developmental neuroscience. Researchers can use the marker to visualize postsynaptic structures and examine how their organization changes during neuronal signaling or development. These applications make it a tool for studying the structural dynamics associated with changing neuronal connections.
The marker links postsynaptic localization with dynamic imaging, allowing researchers to examine how neuronal connections change under different biological contexts. In studies of signaling and development, it can reveal changes in synapse-associated structures over time. The same imaging framework can also support investigations of alterations in synaptic organization associated with disease-related processes.