Riboprobe Detection
Riboprobe Detection - Related Videos
06:19
In situ Protocol for Butterfly Pupal Wings Using Riboprobes
0 Views •
Cited by 8 •
2007
In order to examine gene expression in the pupal wing tissue of Bicyclus anynana, we present an optimized protocol for in situ hybridizations using riboprobes. We also provide guidelines for the further optimization of this protocol for use in pupal wings of other Lepidopteran species.
09:10
Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
0 Views •
Cited by 17 •
2010
This protocol describes a simple adhesive-tape-based approach for sampling of tomato and other fresh produce surfaces, followed by rapid whole cell detection of Salmonella using fluorescence in situ hybridization (FISH).
08:00
Whole-Mount In Situ Hybridization
0 Views •
2023
Whole-mount in situ hybridization (WMISH) is a common technique used for visualizing the location of expressed RNAs in embryos. In this process, synthetically produced RNA probes are first complementarily bound, or "hybridized," to the transcripts of target genes. Immunohistochemistry or fluorescence is then used to detect these RNA hybrids, revealing spatial and temporal patterns of gene expression. Unlike traditional in situ hybridization techniques, which require thin tissue sections whose...
09:00
Detection of Protein Ubiquitination
0 Views •
Cited by 96 •
2009
Ubiquitination is a key posttranslational modification carried out by a set of three enzymes. Mutations of genes involved in this modification are associated with many different human diseases. Here, we describe protocols to detect protein ubiquitination in cultured cells in vivo and test tubes in vitro.
09:08
Detecting Reactive Oxygen Species
0 Views •
2023
Reactive oxygen species are chemically active, oxygen-derived molecules capable of oxidizing other molecules. Because of their reactive nature, there are many deleterious effects associated with unchecked ROS production, including structural damage to DNA and other biological molecules. However, ROS can also be mediators of physiological signaling. There is accumulating evidence that ROS play significant roles in everything from activation of transcription factors to the mediation of...
FAQs
Sequence complementarity determines which transcript can be recognized: the riboprobe binds when its RNA sequence is complementary to the target nucleic acid sequence. This pairing provides the method’s selectivity, allowing researchers to distinguish a transcript of interest from other nucleic acids in the same biological sample. The resulting signal therefore reflects sequence-specific recognition.
The label determines how the bound probe becomes visible or measurable after hybridization and washing. Radioactive, fluorescent, and enzymatic labels offer different signal modalities, so the same sequence-recognition principle can support visual localization or quantitative assessment. Choosing among them affects the form of the readout and how researchers interpret transcript presence in cells, tissues, or samples.
Hybridization creates the opportunity for the labeled probe to pair with a complementary target, but detection occurs only after the sample undergoes the specified washing step. Washing is therefore linked to readout rather than being an optional cleanup stage. The final signal depends on completing both stages so that sequence recognition can be translated into an observable result.
At a basic level, the workflow begins with a biological sample, applies a labeled RNA probe, allows complementary sequences to bind, performs appropriate washing, and then detects the resulting signal. This sequence connects the molecular recognition event to an image or measurement. It can be applied to cells, tissues, or other biological samples, depending on the research question.
Riboprobe detection can provide both spatial and expression-related information. In cells or tissues, the location of a signal indicates where a target RNA transcript is distributed, while the strength or measured readout can support assessment of gene expression. Considering these outputs together helps connect transcript presence with cellular organization and biological function.
These applications use the method to follow where specific RNA transcripts occur and how gene expression changes across biological conditions. During development, it can reveal changing transcript distribution; in infection or disease, it can help examine altered expression patterns. Such comparisons connect sequence-specific molecular signals with broader changes in cellular function and regulation.