Rna Methyltransferase
Rna Methyltransferase - Related Videos
08:31
Antibody-Free Assay for RNA Methyltransferase Activity Analysis
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2019
Here, an antibody-free in vitro assay for direct analysis of methyltransferase activity on synthetic or in vitro transcribed RNA is described.
09:33
In Vitro Assay to Measure Phosphatidylethanolamine Methyltransferase Activity
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Cited by 2 •
2016
The present report describes an in vitro enzymatic assay to measure phosphatidylethanolamine methyltransferase activity using Leishmania cell extracts. This assay is based on the transfer of a radioactive methyl group from S-[Methyl-3H]adenosyl-L-methionine onto endogenous phosphatidylethanolamine.
12:36
In vitro tRNA Methylation Assay with the Entamoeba histolytica DNA and tRNA Methyltransferase Dnmt2 (Ehmeth) Enzyme
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Cited by 20 •
2010
This protocol describes the preparation of a synthetic tRNA substrate for the Entamoeba histolytica DNA/tRNA methyltransferase 2 (Dnmt2) homolog Ehmeth and the measure of its methyltransferase activity. This experimental approach can be used for investigating the activity of other Dnmt2 proteins.
17:12
Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry (CCMS)
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Cited by 13 •
2010
Capture Compounds are trifunctional small molecules to reduce the complexity of the proteome by functional reversible small molecule-protein interaction followed by photo-crosslinking and purification. Here we use a Capture Compound with S-adenosyl-L-homocysteine-binding as selectivity function to isolate methyltransferases from an Escherichia coli whole cell lysate and identify them by MS.
09:54
RNA-Seq
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2023
Among different methods to evaluate gene expression, the high-throughput sequencing of RNA, or RNA-seq. is particularly attractive, as it can be performed and analyzed without relying on prior available genomic information. During RNA-seq, RNA isolated from samples of interest is used to generate a DNA library, which is then amplified and sequenced. Ultimately, RNA-seq can determine which genes are expressed, the levels of their expression, and the presence of any previously unknown transcripts.
FAQs
S-adenosylmethionine serves as the methyl donor during the modification reaction. The enzyme transfers its methyl group to a selected RNA nucleotide, producing a chemically modified base. This donor-dependent step links enzyme activity to the creation of RNA marks that can later influence RNA behavior, including stability, splicing, localization, translation, and interactions with RNA-binding proteins.
Target selection depends on recognition of defined RNA sequences or structures rather than random modification of every transcript. This selectivity allows an enzyme to act on particular nucleotides within particular RNA contexts. Because sequence and structural recognition determine where methyl groups are added, changes in RNA context can influence which molecules receive the modification.
RNA methyltransferases can modify bases such as adenosine or cytosine, and the resulting chemical changes may affect RNA in several ways. A modification can alter stability, splicing, localization, translation, or binding by RNA-associated proteins. Consequently, the modified nucleotide and its surrounding RNA context help determine how the mark influences post-transcriptional regulation.
Investigating these enzymes reveals how gene expression is regulated after RNA has been produced. Researchers can examine how targeted RNA modifications affect transcript stability, processing, cellular localization, translation, and interactions with RNA-binding proteins. This perspective connects nucleotide-level chemical changes with broader control of RNA function and helps clarify post-transcriptional regulation in biology.
RNA methyltransferase research is relevant to diverse biological contexts, including development, viral infection, and cancer. In each area, investigators can consider whether altered RNA modification influences RNA behavior or gene expression. These studies help connect methylation-dependent regulation with cellular responses and with biological states in which RNA control may change.
Abnormal RNA modification is one reason these enzymes are considered in therapeutic research. Studying methyltransferase activity can help identify how disrupted RNA regulation relates to disease-associated biology, including cancer or infection. This work may support strategies that target abnormal RNA modification, while also clarifying which RNA functions and gene-expression processes are affected.