Self-assembled Monolayer
Self-assembled Monolayer - Related Videos
10:27
Antifouling Self-assembled Monolayers on Microelectrodes for Patterning Biomolecules
0 Views •
Cited by 3 •
2009
We present a procedure for forming a poly(ethylene glycol) self-assembled monolayer (PEG-SAM) on a silicon substrate with gold microelectrodes. The PEG-SAM is formed in a single step and prevents biofouling on silicon and gold surfaces. Electrophoresis is then used for patterning biomolecules down to the nanoscale.
14:18
Synthesis, Assembly, and Characterization of Monolayer Protected Gold Nanoparticle Films for Protein Monolayer Electrochemistry
0 Views •
Cited by 1 •
2011
Alkanethiolate stabilized gold colloids known as monolayer protected clusters (MPCs) are synthesized, characterized, and assembled into thin films as an adsorption interface for protein monolayer electrochemistry of simple redox protein like Pseudomonas aeruginosa azurin (AZ) and cytochrome c (cyt c).
08:09
A Technique to Functionalize and Self-assemble Macroscopic Nanoparticle-ligand Monolayer Films onto Template-free Substrates
0 Views •
2014
A simple, robust and scalable technique to functionalize and self-assemble macroscopic nanoparticle-ligand monolayer films onto template-free substrates is described in this protocol.
10:23
Covalent Binding of BMP-2 on Surfaces Using a Self-assembled Monolayer Approach
0 Views •
Cited by 8 •
2013
We describe a method for accomplishing efficient immobilization of BMP-2 on surfaces. Our approach is based on the formation of a self-assembled monolayer to achieve the covalent binding of BMP-2 via its free amine residues. This method is a useful tool to study signaling at the cell membrane.
06:48
Synthesis and Characterization of Self-Assembled Metal-Organic Framework Monolayers Using Polymer-Coated Particles
0 Views •
2024
A protocol for the synthesis and characterization of self-assembled metal-organic framework monolayers is provided using polymer-grafted, metal-organic framework (MOF) crystals. The procedure shows that polymer-grafted MOF particles can be self-assembled at an air-water interface resulting in well-formed, free-standing, monolayer structures as evidenced by scanning electron microscopy imaging.
FAQs
Organization depends on two linked interactions: the head group must associate strongly with the selected solid substrate, while neighboring molecular chains align through noncovalent interactions. This combination helps produce an ordered molecular layer rather than randomly distributed surface-bound molecules. The resulting arrangement gives researchers a reproducible way to control how the surface interacts with surrounding biological or material environments.
The exposed terminal groups define the properties presented at the outer surface. By influencing wettability, charge, and biomolecular compatibility, they can alter whether proteins, nucleic acids, peptides, antibodies, or cells interact favorably with the material. Consequently, changing the terminal chemistry provides a molecular-level means to tune surface behavior without changing the underlying substrate.
The substrate must support a suitable interaction with the molecules used to build the layer. A key example is the strong association between thiol groups and gold, which helps anchor the molecules at the surface. Selecting compatible binding chemistry is therefore important for producing a stable, organized interface on devices such as electrodes, nanoparticles, and sensor surfaces.
A practical sequence begins by selecting the substrate, choosing molecules with a compatible surface-binding head group, and allowing those molecules to adsorb onto the material. Their chains then organize through noncovalent interactions, leaving the chosen terminal groups exposed. The resulting surface can be prepared for biomolecule immobilization or for testing protein and cell attachment.
These layers functionalize device and particle surfaces by placing selected chemical groups at the interface. On electrodes, nanoparticles, and sensor surfaces, the exposed groups can support immobilization of DNA, peptides, or antibodies. This controlled presentation helps create platforms for biosensing, where biological recognition elements are positioned at a material surface for interaction with the surrounding sample.
Surface modification can regulate how proteins or cells attach to a material, making these layers useful for examining cell-material interactions. In tissue engineering, researchers can use the controlled interface to study how surface chemistry affects biological attachment. The same strategy also supports biosensing and the immobilization of antibodies, peptides, or DNA on engineered surfaces.