Stem Cell Biology
Stem Cell Biology - Related Videos
11:19
An Introduction to Stem Cell Biology
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2023
Cells that can differentiate into a variety of cell types, known as stem cells, are at the center of one of the most exciting fields of science today. Stem cell biologists are working to understand the basic mechanisms that regulate how these cells function. These researchers are also interested in harnessing the remarkable potential of stem cells to treat human diseases. Here, JoVE presents an introduction to the captivating world of stem cell biology. We begin with a timeline of landmark...
22:06
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
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Cited by 8 •
2007
This protocol details the derivation of transplantable hematopoietic stem cells from mouse embryonic stem cells (ESC) and their subsequent injection into lethally irradiated recipient mice. Briefly, ESC are differentiated as embryoid bodies, which are then infected with retroviral HoxB4 and co-cultured with OP9 stromal cells and hematopoietic cytokines.
09:48
Development of Organoids from Mouse Pituitary as In Vitro Model to Explore Pituitary Stem Cell Biology
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Cited by 11 •
2022
The pituitary gland is the key regulator of the body's endocrine system. This article describes the development of organoids from the mouse pituitary as a novel 3D in vitro model to study the gland's stem cell population of which the biology and function remain poorly understood.
10:24
Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells
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Cited by 26 •
2011
Despite recent advancements in genetic modification, transfection of human embryonic stem cells (HESCs) remains a capricious process. To our knowledge, systematic and efficient methods to transfect human induced pluripotent stem cells (iPSCs) have not been reported. Here, we describe robust protocols to efficiently transfect and nucleofect human iPSCs.
04:42
Micro-scale Engineering for Cell Biology
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2007
FAQs
Intrinsic gene-regulatory networks help determine how a stem cell maintains its state or moves toward a specialized fate. Signals from the surrounding cellular niche provide additional information, allowing the cell to respond to local conditions. Studying both influences is important because stem cell behavior reflects the interaction between internal regulatory programs and external cellular signals.
The type of cell division affects how stem cell populations are maintained while producing differentiated descendants. Symmetric division can distribute similar cell fates between daughter cells, whereas asymmetric division can separate self-renewal from commitment. Examining this balance helps explain how tissues support ongoing maintenance without exhausting their stem cell populations.
Potency depends on coordinated regulation rather than a single signal. Intrinsic gene-regulatory networks interact with cues from the surrounding niche to balance continued self-renewal with commitment. Researchers therefore examine both cellular programs and environmental signals when studying how stem cells respond to conditions and how their developmental choices are controlled.
Researchers use embryonic, adult, and induced pluripotent stem cells as experimental systems for studying development and investigating disease mechanisms. These models provide a way to examine how cells maintain potency, respond to signals, and produce differentiated cell types. Comparing stem cell systems can therefore connect developmental processes with changes associated with disease.
Stem cell research provides systems in which scientists can test drugs while examining cellular behavior and differentiation-related processes. Because stem cells can generate differentiated cell types, they can help researchers investigate how treatments affect biologically relevant cells in experimental models. This application links fundamental studies of cell regulation with evaluation of potential therapeutic strategies.
Understanding how stem cells preserve potency and respond to niche signals may support the development of regenerative therapies and tissue-engineering approaches. This knowledge can help guide efforts to produce appropriate differentiated cell types and maintain useful cellular behavior. The field also contributes to personalized medicine by connecting stem cell models with an individual’s disease or treatment context.