Fibroblast Cell Suspension
Fibroblast Cell Suspension - Related Videos
03:32
Generating Neurons by Reprogramming Human Dermal Fibroblast Cells
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2025
The video demonstrates the generation of neurons from adult human dermal fibroblasts (aHDFs). The aHDFs are reprogrammed using a lentiviral vector carrying genes for neuronal transcription factors and short hairpin RNAs (shRNAs). The shRNAs inhibit the expression of a neuronal transcriptional repressor, and the neuronal transcription factors activate genes to differentiate aHDFs into neurons. These reprogrammed aHDFs are cultured in an early and a late neuronal conversion medium to generate...
15:43
Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts
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Cited by 25 •
2011
Carcinoma-associated fibroblasts (CAFs) rich in myofibroblasts present within the tumour stroma, play a major role in driving tumour progression. We developed a coimplantation tumour xengraft model for experimentally generating CAFs from human mammary fibroblasts. The protocol describes how to establish CAF myofibroblasts that acquire an ability to promote tumourigenesis.
10:04
Establishment of Proliferative Tetraploid Cells from Nontransformed Human Fibroblasts
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Cited by 2 •
2017
Although proliferative polyploid cells are necessary to analyze chromosomal instability of polyploid cells, creating such cells from nontransformed human cells is not easy. The present report describes relatively simple procedures to establish proliferative tetraploid cells free of a diploid population from normal human fibroblasts.
08:45
Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts
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Cited by 9 •
2016
Here, a new method that allows the conversion of adult skin fibroblasts into insulin-secreting cells is presented. This technique is based on epigenetic conversion, does not involve the use of retroviral vectors nor the acquisition of a stable pluripotent state. It is therefore highly promising for translational medicine applications.
13:58
Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells
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Cited by 38 •
2015
Generation of induced pluripotent stem cells provides fascinating prospects for the derivation of autologous transplants. However, progression through a pluripotent state and laborious re-differentiation still hinders clinical translation. Here we describe the derivation of adult human fibroblasts and their direct conversion into induced neural progenitor cells and the subsequent differentiation into neural lineages.
FAQs
Mechanical or enzymatic dissociation separates fibroblasts from one another so they can be resuspended and handled as a defined cell population. This step creates a more uniform starting material for seeding assays or combining fibroblasts with tumor cells. Maintaining viability during dissociation is important because damaged cells may not provide a reliable representation of stromal behavior.
A defined cell concentration allows researchers to introduce comparable numbers of fibroblasts into separate experimental conditions. Standardization improves reproducibility when comparing assays, co-cultures, or treatment responses, because differences are less likely to result from unequal fibroblast input. It also supports controlled examination of how stromal cell abundance influences interactions with tumor cells.
Within cancer research models, fibroblasts provide a stromal component that can be examined alongside tumor cells. Their cell-cell signaling and extracellular matrix production offer ways to study how the surrounding cellular environment may shape tumor-associated behavior. A suspension format helps researchers introduce fibroblasts at a controlled starting point when establishing these combined experimental systems.
Fibroblast preparations can be incorporated into assays designed to examine responses to treatment in the presence of stromal cells. Comparing tumor cells alone with systems that also contain fibroblasts may reveal effects associated with cell-cell signaling or extracellular matrix production. These experiments help place treatment observations in a tumor-microenvironment context rather than evaluating tumor cells in isolation.
The workflow begins by dispersing the fibroblasts through mechanical or enzymatic dissociation. The resulting cells are then resuspended in liquid culture medium at a defined concentration, with attention to maintaining viability. Researchers can seed the preparation into an assay or combine it with tumor cells for co-culture, creating a controlled starting condition for downstream analysis.
The dissociation approach, the choice of liquid culture medium, the final cell concentration, and fibroblast viability are central preparation factors described for this system. Together, they determine whether cells can be handled consistently and introduced into experiments in a controlled manner. Attention to these variables supports comparable seeding across samples and more interpretable assay results.
It is useful when an experiment needs fibroblasts to be added reproducibly to a model containing tumor cells. The preparation supports investigation of stromal contributions, including signaling between cell types and extracellular matrix production. Such co-culture systems can help researchers assess tumor biology or treatment-related effects under conditions that include a relevant connective-tissue cellular component.
These models can show how fibroblasts influence experimental outcomes through interactions with tumor cells, cell-cell signaling, and extracellular matrix production. They can also support comparisons of treatment responses in systems with or without the fibroblast component. The resulting observations connect fibroblast behavior to the broader tumor microenvironment and help interpret cancer-related processes in a stromal context.