Ksfm Culture
Ksfm Culture - Related Videos
05:36
Human Skin Keratinocyte Isolation: A Method to Culture Primary Keratinocytes from Skin Samples
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2023
This video describes the isolation of keratinocytes—the most prominent cells of human skin's epidermal layer, by employing a combination of enzymatic digestion and mechanical disruption methods. This method helps in the preferential growth of keratinocytes over other skin cell types using a selective growth medium.
08:06
Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney
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Cited by 20 •
2011
In this report we describe a method for the isolation and culture of the progenitor cell niche from the embryonic mouse kidney that can be used to study signaling pathways regulating stem/progenitor cells of the developing kidney. These cultured cells are highly accessible to small molecule and recombinant protein treatment, and importantly also to viral transduction, which allows efficient manipulation of candidate pathways.
08:45
Method for Culture of Early Chick Embryos ex vivo (New Culture)
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Cited by 9 •
2008
This video demonstrates New culture, a method by which chick embryos are cultured outside the egg for up to 24 hr. This method enables one to study early development (primitive streak to 14 som.), a period corresponding to E7-9 in mouse. Applications of this technique include electroporation, in situ hybridization and immunohistochemistry.
10:41
Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles
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Cited by 16 •
2015
This protocol describes the primary culture/co-culture of mouse ovarian tissue, using ovaries from neonatal mice and individual ovarian follicles from prepubertal mice. The culture techniques support development in a highly physiological manner, allowing investigation of the effect of extrinsic agents on the ovary, and of interactions between ovarian follicles.
08:47
Culturing Mouse Cardiac Valves in the Miniature Tissue Culture System
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Cited by 10 •
2015
Here, we present an ex vivo flow model in which murine cardiac valves can be cultured allowing the study of the biology of the valve.
FAQs
The serum-free formulation reduces undefined biological influences that may otherwise affect proliferation, differentiation, and signaling. Because the medium supplies essential nutrients and selected growth-promoting components in a more controlled setting, investigators can attribute differences between experimental groups more confidently to the cells or treatment being studied. This supports clearer interpretation of epithelial cell behavior.
Formulation control improves reproducibility by limiting variation from poorly defined serum constituents. Consistent medium conditions allow investigators to compare cell growth, differentiation, signaling, and treatment responses across experiments with fewer uncontrolled influences. In cancer research, this is especially useful when distinguishing intrinsic differences between normal epithelial cells and transformed cells.
Researchers can maintain normal and transformed epithelial cells under comparable controlled conditions, then compare their growth regulation, signaling, differentiation, or responses to experimental treatments. The shared culture environment helps separate cell-state differences from medium-related variation. Such comparisons provide a framework for examining changes associated with oncogenic transformation and epithelial tumor biology.
This culture system supports investigation of proliferation, differentiation, signaling, growth regulation, and responses to drugs or other experimental treatments. Studying these behaviors under defined conditions helps researchers evaluate how oncogenic changes alter epithelial cell properties. The resulting comparisons can clarify relationships between cellular state, environmental conditions, and treatment response.
A study can begin by maintaining the selected epithelial cell populations in KSFM under controlled conditions, followed by comparison of normal and transformed cells or treated and untreated groups. Investigators then assess outcomes such as proliferation, differentiation, signaling, growth regulation, or drug response. Keeping the culture conditions consistent is central to interpreting those comparisons.
KSFM culture is useful when investigators need to compare how normal and transformed epithelial cells respond to a drug under the same defined medium conditions. The system can reduce variation from undefined serum factors, making differences in observed responses easier to evaluate. These comparisons may help connect treatment effects with oncogenic changes or altered growth regulation.
KSFM culture provides a controlled two-dimensional or maintenance context for examining epithelial cell behavior, while three-dimensional models can extend those investigations to more complex tumor biology. Using both approaches allows researchers to compare defined cellular responses with findings from models that represent additional aspects of the cellular environment. Together, they can strengthen interpretation of epithelial cancer studies.