Active Flow Technology
Active Flow Technology - Related Videos
13:59
Reduced-gravity Environment Hardware Demonstrations of a Prototype Miniaturized Flow Cytometer and Companion Microfluidic Mixing Technology
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Cited by 9 •
2014
Spaceflight blood diagnostics need innovation. Few demonstrations have been published illustrating in-flight, reduced-gravity health diagnostic technology. Here we present a method for construction and operation of a parabolic flight test rig for a prototype point-of-care flow-cytometry design, with components and preparation strategies adaptable to other setups.
08:01
Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology
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Cited by 50 •
2012
A rapid and accurate point-of-care test for invasive pulmonary aspergillosis is presented. It takes advantage of lateral-flow technology using a specific monoclonal antibody that binds to an Aspergillus antigen secreted during pulmonary infections. The assay is compatible with serum and brochoalveolar lavage and represents a novel adjunct test for disease diagnosis.
17:07
Flow Cytometry and Fluorescence-Activated Cell Sorting (FACS): Isolation of Splenic B Lymphocytes
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2023
Source: Perchet Thibaut1,2,3, Meunier Sylvain1,2,3, Sophie Novault4, Rachel Golub1,2,3 1 Unit for Lymphopoiesis, Department of Immunology, Pasteur Institute, Paris, France 2 INSERM U1223, Paris, France 3 Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Paris, France 4 Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute, Paris, France The overall function of the immune system is to defend the body against infectious...
05:37
Analyzing Basophil Activation in the Presence of a Test Allergen using Flow Cytometry
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2025
In this video, we demonstrate the test to evaluate basophil activation in the presence of a test allergen using flow cytometry.
11:37
Measuring Calpain Activity in Fixed and Living Cells by Flow Cytometry
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Cited by 6 •
2010
This article will detail the protocol for measuring calpain activity in fixed and living cells using flow cytometry.
FAQs
These configurations divide or redirect eluent through the packed stationary phase instead of allowing a single conventional axial path. Managing movement in this way can reduce band broadening and wall effects, which helps solute bands remain narrower as they travel through the column. The resulting improvement may appear as sharper peaks and higher separation efficiency.
Wall effects can contribute to uneven movement near the column boundary, encouraging band spreading and reducing the quality of separation. Active Flow Technology addresses this concern through controlled flow-path designs that redirect eluent within the packed bed. Limiting this source of broadening can improve peak shape and support more reliable resolution when analyzing complex chemical mixtures.
Conventional axial-flow columns primarily move mobile phase along the column axis, whereas Active Flow Technology uses configurations that divide or redirect flow through the packed bed. This design difference changes how eluent interacts with the stationary phase and the column walls. The intended outcome is lower band broadening, improved peak shape, and more efficient chromatographic separation.
The selected flow-path configuration is an important consideration because Active Flow Technology supports radial or parallel segmented designs rather than one fixed arrangement. Researchers can use this flexibility during analytical method development to match the column design to the separation problem. Evaluating peak shape, separation efficiency, and quantitative performance helps identify the most useful configuration for a chemically complex sample.
During method development, the technique provides a flexible column-design option for examining how controlled flow paths affect chromatographic behavior. Researchers can compare separation efficiency, peak shape, and quantitative performance while working with a target mixture. This information helps determine whether a radial or parallel segmented configuration offers a useful advantage for the intended liquid chromatography analysis.
The approach is relevant when liquid chromatography must resolve complex mixtures with high accuracy. Pharmaceutical samples may benefit from improved resolution and quantitative performance, while environmental analyses can use the same design principles for chemically complicated sample compositions. Its broader value lies in supporting high-resolution analysis across applications where peak shape and efficient separation affect interpretation.