Ectodermal Cover

Ectodermal cover is the outer embryonic layer that forms a protective covering over the developing embryo and contributes to several specialized tissues. During development, signaling interactions between the ectoderm and neighboring germ layers direct cells toward surface ectoderm or neural ectoderm fates; these populations then undergo coordinated differentiation, migration, and morphogenesis. The ectodermal cover gives rise primarily to the epidermis and its appendages, while related ectodermal tissues form the nervous system and sensory structures. Studying its formation helps explain epithelial organization, neural induction, tissue patterning, and congenital disorders involving skin, hair, teeth, or neural development.

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Research

JoVE Journal - Biology

Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme

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Cited by 23 •

2013

A protocol for separation of embryo facial ectoderm and mesenchyme is described. We use Dispase II to treat whole embryos first, dissect whole facial prominences out, and then separate the facial ectoderm and mesenchyme.

Assessing Signaling Properties of Ectodermal Epithelia During Craniofacial Development

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Cited by 7 •

2011

This article describes a tissue transplantation technique that was designed to test the signaling and patterning properties of surface cephalic ectoderm during craniofacial development.

Polarized Translocation of Fluorescent Proteins in Xenopus Ectoderm in Response to Wnt Signaling

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Cited by 2 •

2011

Xenopus embryonic ectoderm has become an attractive model for studies of cell polarity. An assay is described, in which subcellular distribution of fluorescent proteins is assessed in ectoderm cells. This protocol will help address questions related to spatial control of signaling.

Fat-Covered Islet Transplantation Using Epididymal White Adipose Tissue

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Cited by 3 •

2021

This fat-covered islet transplantation method is suitable for the detection of engrafted islets in the intraperitoneal cavity. Notably, it does not require the use of biobinding agents or suturing.

How to Create Conditioned Taste Aversion for Grazing Ground Covers in Woody Crops with Small Ruminants

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Cited by 4 •

2016

Feeding behavior preferences in livestock can be modified to implement a grazing management plan in woody crops. Here, we present a protocol to show a lithium chloride dose after eating a new plant that induces conditioned taste aversion.

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