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Polarized Translocation of Fluorescent Proteins in Xenopus Ectoderm in Response to Wnt Signaling
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Polarized Translocation of Fluorescent Proteins in Xenopus Ectoderm in Response to Wnt Signaling

Article DOI: 10.3791/2700 • 06:56 min • May 26th, 2011
May 26th, 2011 •
1, 1
1Department of Developmental and Regenerative Biology, Mount Sinai School of Medicine

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Xenopus embryonic ectoderm has become an attractive model for studies of cell polarity. An assay is described, in which subcellular distribution of fluorescent proteins is assessed in ectoderm cells. This protocol will help address questions related to spatial control of signaling.

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Polarized Translocation Fluorescent Proteins Xenopus Ectoderm Wnt Signaling Cell Polarity Embryonic Development Signaling Pathways Molecular Components Subcellular Localization Protein Localization RNA Microinjection Ectodermal Explants Epifluorescence Diversin Cytoplasmic Protein Cell Polarity Determination Wnt Signal Transduction Wnt Ligand Frizzled Receptor
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