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Chapters
- 00:05Title
- 01:00Introduction
- 01:27In vitro Fertilization of Xenopus Eggs
- 02:34RNA Microinjection
- 03:46Prepare Ectodermal Explants for Imaging
- 04:46Alternative Approach: Prepare Cryosectioned Embryos for Imaging
- 05:57Representative Fluorescent Imaging Results
- 06:29Conclusion
Xenopus embryonic ectoderm has become an attractive model for studies of cell polarity. An assay is described, in which subcellular distribution of fluorescent proteins is assessed in ectoderm cells. This protocol will help address questions related to spatial control of signaling.
Tags
Polarized TranslocationFluorescent ProteinsXenopus EctodermWnt SignalingCell PolarityEmbryonic DevelopmentSignaling PathwaysMolecular ComponentsSubcellular LocalizationProtein LocalizationRNA MicroinjectionEctodermal ExplantsEpifluorescenceDiversinCytoplasmic ProteinCell Polarity DeterminationWnt Signal TransductionWnt LigandFrizzled Receptor
