Molecular Pathways
Molecular Pathways - Related Videos
08:23
Real-Time Impedance-based Cell Analyzer as a Tool to Delineate Molecular Pathways Involved in Neurotoxicity and Neuroprotection in a Neuronal Cell Line
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Cited by 2 •
2014
Improved in vitro neurotoxicity assays would aid the identification of new neuroprotective compounds. The utility of a real-time impedance-based cell analyzer to determine cytotoxicity and cytoprotection in neuronal cell lines and to delineate the involvement of second messenger pathways, thus gaining insight in the mechanism of neuroprotection is presented.
09:55
Molecular Cloning
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2023
Molecular cloning is a set of methods, which are used to insert recombinant DNA into a vector - a carrier of DNA molecules that will replicate recombinant DNA fragments in host organisms. The DNA fragment, which may be a gene, can be isolated from a prokaryotic or eukaryotic specimen. Following isolation of the fragment of interest, or insert, both the vector and insert must be cut with restriction enzymes and purified. The purified pieces are joined together though a technique called...
10:22
Use of the TetON System to Study Molecular Mechanisms of Zebrafish Regeneration
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Cited by 10 •
2015
Here we outline the workflow for using the TetON system to achieve tissue-specific gene expression in the adult regenerating zebrafish tail fin.
12:02
Molecular Evolution of the Tre Recombinase
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Cited by 4 •
2008
Here we report the generation of Tre recombinase through directed, molecular evolution. Tre recombinase recognizes a pre-defined target sequence within the LTR sequences of the HIV-1 provirus, resulting in the excision and eradication of the provirus from infected human cells. While still in its infancy, directed molecular evolution will allow the creation of custom enzymes that will serve as tools of molecular surgery and molecular medicine.
11:25
An Introduction to Cellular and Molecular Neuroscience
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2023
Cellular and molecular neuroscience is one of the newest and fastest growing subdisciplines in neuroscience. By investigating the influences of genes, signaling molecules, and cellular morphology, researchers in this field uncover crucial insights into normal brain development and function, as well as the root causes of many pathological conditions. This video introduction to the fascinating world of cellular and molecular neuroscience begins with a timeline of landmark studies, from the...
FAQs
A variant can affect a pathway component, the amount of gene expression, the timing of activation, or interactions among regulatory molecules. These changes may disrupt downstream protein production, modification, or cellular responses. As a result, the same pathway can contribute differently to metabolism, development, or disease depending on which component is altered and how that alteration propagates through the network.
The order and timing of transcription, RNA processing, translation, and protein modification determine when cellular functions become active. A change in timing can therefore modify a response even when the pathway components remain present. Examining timing alongside molecular interactions helps explain how genetic differences influence development, cellular behavior, and the progression of pathway-related disorders.
An isolated gene view focuses on one gene or product, whereas pathway analysis considers coordinated relationships among genes, regulatory molecules, proteins, and cellular responses. This network perspective can reveal how a change in one component affects several downstream processes. It is especially useful when a genetic trait or disease cannot be explained by a single gene acting independently.
A pathway map can be organized by tracing how gene activation or an external signal leads through transcription, RNA processing, translation, protein modification, and molecular interactions. Researchers then connect these steps to the resulting cellular function or trait. Relating pathway components to genetic variants helps identify points where altered regulation may change the overall outcome.
Pathway analysis places individual variants within the broader sequence of molecular events they may influence. Researchers can examine whether a change affects gene expression, a regulatory interaction, protein production, or a later cellular response. This approach supports interpretation of how genetic variation may contribute to altered metabolism, development, disease mechanisms, or other cellular traits.
They are useful when researchers need to connect altered genes with the cellular functions and responses associated with disease. Mapping the affected sequence can show where regulation, protein production, modification, or molecular interaction changes. The resulting view may help explain disease mechanisms, organize related genetic findings, and identify pathway components suitable for diagnostic marker or therapeutic-target evaluation.
Molecular pathways provide a framework for connecting individual genes to larger cellular networks rather than analyzing each gene in isolation. This network-level organization helps researchers relate gene activity, regulatory interactions, cellular functions, and traits. In systems biology, such connections can clarify how multiple pathway components jointly shape cellular behavior and how genetic changes influence broader network responses.