Chemical Compound Screening
Chemical Compound Screening - Related Videos
07:25
High-throughput Screening of Chemical Compounds to Elucidate Their Effects on Bacterial Persistence
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Cited by 1 •
2021
In this method paper, we present a high-throughput screening strategy to identify chemical compounds, such as osmolytes, that have a significant impact on bacterial persistence.
09:44
High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
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2015
Here we describe a cell-based reporter gene assay as a valuable tool to screen chemical libraries for compounds modulating post-transcriptional control mechanisms exerted through 3’ UTR.
11:34
High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
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Cited by 14 •
2014
In vitro assays to measure virus replication have been greatly improved by the development of recombinant RNA viruses expressing luciferase or other enzymes capable of bioluminescence. Here we detail a high-throughput screening pipeline that combines such recombinant strains of measles and chikungunya viruses to isolate broad-spectrum antivirals from chemical libraries.
10:49
Development of automated imaging and analysis for zebrafish chemical screens.
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Cited by 48 •
2010
We report the development of a system for automated imaging and analysis of zebrafish transgenic embryos in multiwell plates. This demonstrates the ability to measure dose dependent effects of a small molecule, BCI, on Fibroblast Growth Factor reporter gene expression and provide technology for establishing high-throughput zebrafish chemical screens.
02:57
A Fluorescence-Based Assay Using Engineered Lymphocytes for Screening Immunomodulatory Compounds
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2025
This video demonstrates the screening of immunomodulatory compounds via fluorescent-based assay. Transgenic mouse-derived T lymphocytes with green fluorescent protein expression cassettes are treated with these compounds. The enhanced and reduced GFP signals enable the identification of the compounds' stimulatory and inhibitory effects, respectively.
FAQs
Testing compounds across defined concentrations reveals whether a biological effect changes with dose, rather than appearing at only one measurement point. Controls provide a reference for interpreting effects on pathogen growth, immune-cell activation, signaling, or inflammatory responses. Together, these comparisons help researchers distinguish a reproducible compound-associated response from variation within the assay.
An initial hit shows that a compound produces a potentially useful effect, but it does not establish whether that effect is specific or suitable for further study. Secondary testing examines activity against the intended biological process while assessing selectivity and toxicity. Characterizing dose-response relationships then clarifies how changing concentration affects the observed response.
Cell-based assays measure compound effects in living cells, including immune-cell activation or inflammatory responses, whereas biochemical assays assess effects in a defined biological system. This distinction helps align the assay with the research question. Screening across these formats can examine pathogen growth, cellular behavior, or signaling, depending on the biological effect being investigated.
The workflow begins by testing candidate small molecules in a cell-based or biochemical assay using defined concentrations and controls. Researchers measure a selected biological response and identify compounds showing the desired effect. Promising hits then proceed to secondary testing, where activity is confirmed and selectivity, toxicity, and dose-response behavior are characterized.
In infection research, screening can identify compounds that affect pathogen growth or alter host responses such as immune-cell activation, signaling, and inflammation. These results help separate candidates with antimicrobial potential from compounds that act as immunomodulators. The measured responses also provide experimental tools for examining interactions between pathogens and their hosts.
The approach supports several complementary goals: discovering antimicrobial agents, identifying compounds that modify immune activity, and finding tools for investigating host-pathogen interactions. Researchers select assays that measure the relevant pathogen or host response, then prioritize hits through confirmation and dose-response analysis. This connects chemical activity with disease-related biological processes in a controlled experimental setting.