Mdr-tb Genotyping
Mdr-tb Genotyping - Related Videos
08:54
Validated LC-MS/MS Panel for Quantifying 11 Drug-Resistant TB Medications in Small Hair Samples
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Cited by 5 •
2020
Current methods of analyzing patients’ adherence to complex drug resistant-tuberculosis (DR-TB) regimens can be inaccurate and resource-intensive. Our method analyzes hair, an easily collected and stored matrix, for concentrations of 11 DR-TB medications. Using LC-MS/MS, we can determine sub-nanogram drug levels that can be utilized to better understand drug adherence.
08:27
Mouse Genotyping
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2023
Even though the human genome was mapped over 10 years ago, scientists are still far from understanding the function of every human gene! One way to evaluate how a gene functions is to disrupt the sequence encoding it and then evaluate the impact of this change (the phenotype) on the animal’s biology. This approach is commonly used in the mouse (Mus musculus), since it shares a high degree of genetic similarity with humans. To track the animals bearing genetic changes over several generations,...
08:23
SNP Genotyping
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2023
Single nucleotide polymorphisms, or SNPs, are the most common form of genetic variation in humans. These differences at individual bases in the DNA often do not directly affect gene expression, but in many cases can still be useful for locating disease-associated genes or for diagnosing patients. Numerous methodologies have been established to identify, or “genotype”, SNPs.JoVE’s introduction to SNP Genotyping begins by discussing what SNPs are and how they can be used to identify...
13:06
Pyrosequencing: A Simple Method for Accurate Genotyping
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Cited by 22 •
2008
Pyrosequencing(R) is one of the most thorough yet simple methods to date used to analyze polymorphisms. This method has led to rapid and efficient single-nucleotide polymorphism evaluation including many clinically relevant polymorphisms. The technique and methodology of Pyrosequencing is explained.
10:41
A Tuberculosis Molecular Bacterial Load Assay (TB-MBLA)
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Cited by 18 •
2020
We describe a tuberculosis molecular bacterial load assay test performed after heat inactivation of sputum. Heat inactivation renders sputum samples noninfectious and obviates the need for containment level 3 laboratories for tuberculosis molecular tests.
FAQs
Resistance-associated mutations provide molecular clues about whether a Mycobacterium tuberculosis strain carries markers linked to reduced susceptibility to important drugs, particularly isoniazid and rifampicin. By examining bacterial DNA, genotyping can connect a genetic change with a resistance pattern. This supports an earlier molecular assessment while also helping researchers compare resistance profiles among strains.
Genotyping and culture-based drug susceptibility testing provide complementary information. Molecular analysis examines resistance-associated genetic markers, whereas culture-based testing evaluates drug response through a conventional laboratory approach. Using both can strengthen interpretation of resistance, because genetic findings provide rapid assessment while culture-based results offer an independent basis for evaluating susceptibility.
Genetic comparisons can show whether tuberculosis isolates share related patterns or differ from one another. Investigators use these relationships to examine possible links among cases and to clarify transmission networks. The approach does not replace epidemiological information, but it adds molecular evidence that can help distinguish connected cases from infections caused by less-related strains.
The workflow begins with bacterial DNA and focuses on genetic markers or genomic regions associated with resistance. Results are then interpreted in relation to the relevant antituberculosis drugs, especially isoniazid and rifampicin, and may be compared across isolates. When combined with conventional susceptibility results, this process supports a more complete resistance assessment.
MDR-TB genotyping is especially useful when researchers need to assess resistance rapidly, investigate a suspected outbreak, or monitor patterns across populations. Comparing strain-related genetic information can help clarify transmission networks, while repeated surveillance can reveal how resistance-associated markers are distributed. These applications support broader efforts to monitor and control multidrug-resistant tuberculosis.
In immunology and infection research, genotyping links bacterial genetic variation with clinically and epidemiologically important infection patterns. It can help researchers examine how resistant strains circulate, identify relationships among cases, and interpret resistance alongside other infection data. The resulting evidence supports treatment planning, transmission analysis, and surveillance without relying solely on one laboratory method.