Mouse Pancreas Islets
Mouse Pancreas Islets - Related Videos
07:04
Effective Isolation of Functional Islets from Neonatal Mouse Pancreas
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Cited by 19 •
2017
We describe a protocol herein for isolating intact islets from neonatal mice. Pancreata were partially digested with collagenase, followed by washing and hand picking. 20 - 80 islet clusters can be obtained per pancreas from newly born mice, which are suitable for several islet studies.
03:40
Intravital Imaging: A Method for Cellular Level Observation of the Mouse Pancreas Through a Stabilized Imaging Window
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2025
In this video, we describe the intravital imaging of a mouse pancreas through a stabilized window for long-term visualization of cellular dynamics.
07:21
Pancreatic Islets Isolation through Collagenase Perfusion: An Enzymatic Method to Isolate Pancreatic Islet Cells
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2023
Pancreatic islets, also called the Islets of Langerhans, are a cluster of endocrine cells which produces hormones for glucose regulation and other important biological functions. This protocol explains a method for isolating pancreatic islets by first perfusing collagenase via Ampulla of Vater to digest the exocrine pancreatic tissue and a simplified density gradient centrifugation to purify the islets from mice.
03:33
Grafting Pancreatic Islets: A Method to Implant Pancreatic Islets into the Anterior Chamber of the Mouse Eye for In Vivo Imaging
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2023
This video describes the protocol for grafting human pancreatic islets into the anterior chamber of the mouse’s eye to monitor revascularization of human islet grafts in vivo. It also helps to understand the contribution of recipient versus donor cells in promoting the encapsulation and vascularization of the graft.
12:33
A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination
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Cited by 54 •
2014
Assaying in vitro β-cell function using isolated mouse islets of Langerhans is an important component in the study of diabetes pathophysiology and therapeutics. While many downstream applications are available, this protocol specifically describes the measurement of intracellular cyclic adenosine monophosphate (cAMP) as an essential parameter determining β-cell function.
FAQs
Communication among endocrine, vascular, and immune cells can influence how islets respond to changing glucose conditions and inflammatory signals. This cellular context matters because immune activity may affect hormone-producing cells directly or alter the surrounding environment that supports their function. Studying these interactions helps connect local immune responses with broader changes in endocrine regulation.
Isolated islets allow researchers to examine how immune responses affect beta-cell integrity and hormone secretion in a controlled experimental setting. Changes in endocrine function can then be considered alongside inflammatory effects, helping distinguish direct cellular injury from broader tissue-level consequences. This makes the model useful for investigating mechanisms associated with diabetes development.
Pathogen-associated effects can be examined for their influence on islet inflammation and endocrine performance. The model provides a way to study how infection-related signals may alter beta-cell function or contribute to tissue injury without relying only on whole-animal observations. Such experiments can clarify links between infectious processes, immune activation, and metabolic disease.
Isolated islet studies emphasize controlled observation of endocrine and immune responses outside the pancreas, whereas transplantation introduces islets into a recipient context. The first approach is suited to examining cellular mechanisms and direct effects, while the second can help assess how immune responses influence islet survival and function in a broader biological setting.
A typical experimental workflow uses isolated mouse islets, maintains them in culture, and then evaluates responses through ex vivo immune assays or related measurements of endocrine function. Researchers can expose the preparation to relevant inflammatory or pathogen-associated conditions and compare outcomes with appropriate controls. This design supports controlled analysis of immune effects on islet activity.
Researchers may select this model when they need to connect immune or infection-related mechanisms with changes in endocrine function. Islet culture supports focused mechanistic experiments, transplantation extends investigation to immune effects in a recipient setting, and ex vivo assays enable direct analysis of immune responses. Together, these applications can inform studies of diabetes development and potential interventions.