Salmonella Shigella Screening
Salmonella Shigella Screening - Related Videos
06:55
A High-throughput Platform for the Screening of Salmonella spp./Shigella spp.
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2018
Salmonella spp./Shigella spp. are common pathogens attributed to diarrhea. Here, we describe a high-throughput platform for the screening of Salmonella spp./Shigella spp. using real-time PCR combined with guided culture.
08:24
Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation
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Cited by 12 •
2020
Loop-mediated isothermal amplification (LAMP) is an isothermal nucleic acid amplification test (iNAAT) that has attracted broad interest in the pathogen detection field. Here, we present a multi-laboratory-validated Salmonella LAMP protocol as a rapid, reliable, and robust method for screening Salmonella in animal food and confirming presumptive Salmonella from culture isolation.
04:56
Epithelial Cell Infection Analyses with Shigella
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Cited by 1 •
2024
The present protocol describes infection assays to interrogate Shigella adherence, invasion, and intracellular replication using in vitro epithelial cell lines.
06:11
A High-throughput Shigella-specific Bactericidal Assay
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Cited by 7 •
2019
Here we present a protocol to measure Shigellacidal activity of antibodies in serum. Serum is mixed with bacteria and exogenous complement, incubated, and the reaction mixture is plated on agar plates. Viable bacteria form colonies which are counted, using an automated colony enumerator, and used to determine the bactericidal titer.
08:56
Imaging Ca2+ Responses During Shigella Infection of Epithelial Cells
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Cited by 3 •
2018
Here, we present protocols to visualize calcium (Ca2+) responses elicited by HeLa cells infected by Shigella. By optimizing the parameters of bacterial infection and imaging with Ca2+ fluorescent probes, atypical global and local Ca2+ signals induced by bacteria over a large range of infection kinetics are characterized.
FAQs
Selective media suppress much of the accompanying flora, making suspected enteric pathogens easier to recover from a mixed clinical specimen. Differential media then reveal characteristic colony reactions that help distinguish potentially relevant organisms from other growth. Using both functions together improves the efficiency of initial examination and directs attention toward colonies requiring further confirmation.
Characteristic colony reactions provide an initial indication that an isolate may belong to Salmonella or Shigella, but they do not by themselves establish the organism’s identity. The workflow therefore treats these findings as presumptive and subjects selected isolates to biochemical or serological confirmation. This sequence reduces the risk of basing clinical or public health decisions on screening appearance alone.
Biochemical and serological confirmation are follow-up approaches applied to presumptive isolates after screening media have identified colonies of interest. Biochemical confirmation evaluates organism-associated reactions, whereas serological confirmation uses antigen-related reactivity to support identification. Their role is to strengthen the interpretation of preliminary colony findings before results inform diagnosis, surveillance, or infection-control actions.
Clinical specimens contain organisms beyond the suspected pathogens, and this accompanying flora can complicate recovery and interpretation. Selective media address that challenge by suppressing much of the unwanted growth, while differential reactions help reveal colonies with relevant characteristics. Consistent use of this strategy allows laboratories to process specimens more efficiently and focus confirmatory work on presumptive isolates.
The workflow begins with inoculation of a clinical specimen onto selective and differential media. Laboratory personnel then examine the resulting growth for characteristic colony reactions and identify presumptive isolates for additional testing. Biochemical or serological confirmation follows, producing information that can support diagnostic interpretation and determine whether broader infection-control or public health follow-up is warranted.
The approach is useful when laboratories need to investigate suspected bacterial gastroenteritis or enteric infection, clarify a clinical diagnosis, or examine possible transmission events. Confirmed identification can also support outbreak investigation, infection-control decisions, and public health surveillance. In these settings, the method connects individual specimen testing with recognition of broader patterns affecting groups or communities.
Within immunology and infection, the workflow supplies organism-identification data from clinical specimens rather than relying only on symptoms or broader clinical categories. Serological confirmation provides an antigen-related follow-up option, while surveillance results help reveal patterns of transmission. Together, these findings support interpretation of host-associated infection events and the public health response to enteric pathogens.