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JoVE Journal
Immunology and Infection

This content is Open Access.

JoVE Journal Immunology and Infection
A High-throughput Shigella-specific Bactericidal Assay
 

A High-throughput Shigella-specific Bactericidal Assay

Article doi: 10.3791/59164
February 27th, 2019 •
1, 2, 1, 2
1Department of Enteric Infections, Bacterial Diseases Branch, Walter Reed Army Institute of Research, 2University of Alabama at Birmingham

Summary
Automatic Translation
February 27th, 2019

Here we present a protocol to measure Shigellacidal activity of antibodies in serum. Serum is mixed with bacteria and exogenous complement, incubated, and the reaction mixture is plated on agar plates. Viable bacteria form colonies which are counted, using an automated colony enumerator, and used to determine the bactericidal titer.

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