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A High-throughput Shigella-specific Bactericidal Assay
A High-throughput <em>Shigella</em>-specific Bactericidal Assay
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
A High-throughput Shigella-specific Bactericidal Assay

A High-throughput Shigella-specific Bactericidal Assay

11,742 Views

06:11 min

February 27, 2019

DOI:

06:11 min
February 27, 2019

11705 Views
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Summary

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Here we present a protocol to measure Shigellacidal activity of antibodies in serum. Serum is mixed with bacteria and exogenous complement, incubated, and the reaction mixture is plated on agar plates. Viable bacteria form colonies which are counted, using an automated colony enumerator, and used to determine the bactericidal titer.

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