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A High-throughput Shigella-specific Bactericidal Assay
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A High-throughput Shigella-specific Bactericidal Assay

A High-throughput Shigella-specific Bactericidal Assay

DOI:
10.3791/59164-v

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06:11 min

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February 27, 2019

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, , ,
1Department of Enteric Infections, Bacterial Diseases Branch,Walter Reed Army Institute of Research, 2University of Alabama at Birmingham

Chapters

  • 00:04Title
  • 00:44Serum Bactericidal Assay (SBA)
  • 04:48Results: Analysis of the Bacterial Assay
  • 05:42Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a protocol to measure Shigellacidal activity of antibodies in serum. Serum is mixed with bacteria and exogenous complement, incubated, and the reaction mixture is plated on agar plates. Viable bacteria form colonies which are counted, using an automated colony enumerator, and used to determine the bactericidal titer.

Tags

Keywords: ShigellaBactericidal AssayHigh-throughputAntibodySerumSerial DilutionsTarget BacteriaComplementBRCPlate ShakerIncubation

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