Subcutaneous Immunization
Subcutaneous Immunization - Related Videos
06:32
Protective Efficacy and Pulmonary Immune Response Following Subcutaneous and Intranasal BCG Administration in Mice
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Cited by 14 •
2016
We herein detail the methodology followed to compare protective efficacy and lung immune response induced by intranasal and subcutaneous immunization with BCG in mouse model. Our results show the benefits of pulmonary vaccination and suggest a role for IL17-mediated response in vaccine-induced protection.
08:32
Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors
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Cited by 9 •
2018
Here we describe a method to separate and enrich components of the tumor immune and non-immune microenvironment in established subcutaneous tumors. This technique allows for the separate analysis of tumor immune infiltrate and non-immune tumor fractions which can permit comprehensive characterization of the tumor immune microenvironment.
03:52
Subcutaneous Injection of Bacterial Antigen to Induce Systemic Inflammation in a Murine Model
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2025
This video demonstrates the induction of systemic inflammation in an anesthetized mouse. The injection of mycobacterial antigen-adjuvant depots triggers cytokine release and localized inflammation. Lymphatic vessels open, enabling immune cell entry into lymph nodes and activation of T cells, which release pro-inflammatory cytokines, leading to widespread inflammation.
07:49
Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model
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Cited by 15 •
2015
This protocol describes a method for the detailed evaluation of leukocyte subsets within the tumor microenvironment in a mouse tumor model. Chemerin-expressing B16 melanoma cells were implanted subcutaneously into syngeneic mice. Cells from the tumor microenvironment were then stained and analyzed by flow cytometry, allowing for detailed leukocyte subset analyses.
01:38
What is the Immune System?
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2019
Overview The immune system comprises diverse biological structures and processes that protect the body from disease. These processes can be classified into innate and adaptive immunity. To work effectively, the immune system needs to detect pathogens by distinguishing the body’s own structures from foreign elements. If this determination fails, autoimmune diseases occur in which the immune system reacts against the body’s own tissue. The Innate Immune System Acts Fast and Non-specifically
FAQs
Following delivery beneath the skin, antigen-presenting cells capture and process the introduced antigen. They then activate lymphocytes in draining lymph nodes, linking the injection site to the development of adaptive immunity. This sequence helps explain how a localized administration can produce broader antibody and cellular responses relevant to protection against infection.
Adjuvants enhance immune stimulation when included with the preparation. Their role is important because the strength of antigen recognition and subsequent lymphocyte activation can influence the antibody and cellular responses that develop. In vaccine design, evaluating adjuvant use can therefore help researchers investigate strategies for generating more effective protective immunity.
The delivery route affects how the preparation reaches antigen-presenting cells and how immune activation proceeds through draining lymph nodes. As a result, subcutaneous immunization can influence both the timing and effectiveness of the resulting immune response. These effects matter when researchers evaluate vaccine performance or compare strategies for stimulating protective immunity.
Evaluation can focus on the antibody response, the cellular response, or both. These outcomes indicate how lymphocyte activation has translated into adaptive immunity after antigen delivery. Studying them helps researchers assess whether a vaccine or immune-stimulating preparation produces the type of response needed for vaccine development, infection prevention, or treatment-oriented immunotherapy.
This route is used for selected vaccines and immunotherapies rather than serving as a universal delivery approach. Researchers may investigate it when they need to evaluate how administration beneath the skin influences immune activation and response development. Its relevance extends across vaccine design, immune-response studies, and strategies intended to prevent or treat infectious disease.
It provides a framework for studying how antigen delivery initiates antibody and cellular immunity through antigen-presenting cells and draining lymph nodes. In infection research, those responses can be examined in relation to disease prevention. In immunology, the same system supports evaluation of vaccine formulations, adjuvant effects, and immune-stimulating treatments.