Cardiac Metabolism Study
Cardiac Metabolism Study - Related Videos
08:16
Neonatal Cardiac Scaffolds: Novel Matrices for Regenerative Studies
0 Views •
Cited by 3 •
2016
In these studies, we provide methodology for novel, neonatal, murine cardiac scaffolds for use in regenerative studies.
11:51
Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy
0 Views •
Cited by 16 •
2016
This manuscript describes the creation of defined engineered cardiac tissues using surface marker expression and cell sorting. The defined tissues can then be used in a multi-tissue bioreactor to investigate mechanisms of cardiac cell therapy in order to provide a functional, yet controlled, model system of the human heart.
06:04
LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism
0 Views •
Cited by 7 •
2016
Here we show isolated human platelets can be used as an accessible ex vivo model to study metabolic adaptations in response to the complex I inhibitor rotenone. This approach employs isotopic tracing and relative quantification by liquid chromatography-mass spectrometry and can be applied to a variety of study designs.
05:41
Metabolic Profile Analysis of Zebrafish Embryos
0 Views •
Cited by 42 •
2013
Zebrafish represent a powerful vertebrate model that has been under-utilised for metabolic studies. Here we describe a rapid way to measure the in vivo metabolic profile of developing zebrafish that allows the comparison of different mitochondrial function parameters between genetically or pharmacologically manipulated embryos, thereby increasing the applicability of this organism.
10:19
An Introduction to Cell Metabolism
0 Views •
2023
In cells, critical molecules are either built by joining together individual units like amino acids or nucleotides, or broken down into smaller components. Respectively, the reactions responsible for this are referred to as anabolic and catabolic. These reactions require or produce energy typically in the form of a “high-energy” molecule called ATP. Together, these processes make up “Cell Metabolism,” and are hallmarks of healthy, living cells.JoVE’s introduction to cell metabolism briefly...
FAQs
The heart can adjust its use of fatty acids, glucose, lactate, and ketone bodies rather than relying on one fuel under every condition. Workload, oxygen availability, and disease state influence this preference. Measuring these shifts helps determine whether cardiomyocytes retain metabolic flexibility or develop an altered energy pattern associated with ischemia, heart failure, diabetes, or hypertrophy.
Mitochondrial oxidative phosphorylation supplies most of the ATP needed for continuous cardiomyocyte contraction. A cardiac metabolism study therefore examines how substrate use connects with mitochondrial energy production, especially when oxygen becomes limited or disease changes normal cardiac function. Identifying disruptions in this relationship can reveal impaired energy generation and support investigations of cardiovascular pathology.
Changes in workload alter the heart’s energy demand, while oxygen availability constrains the mitochondrial pathway that normally produces most cardiac ATP. Disease can further modify these responses and the balance among available substrates. Comparing metabolic behavior across these conditions helps researchers distinguish adaptive fuel selection from changes that may accompany ischemia, heart failure, diabetes, or hypertrophy.
Investigators combine metabolic assays, imaging, and molecular analysis because each approach provides different information about cardiac energy pathways. Metabolic assays evaluate pathway-related activity, imaging examines relevant changes in the heart, and molecular analysis identifies associated biological alterations. Using these methods together gives a broader view than relying on a single measurement when studying normal function or disease.
A study typically begins by selecting a cardiac condition or comparison, then measuring metabolic features with appropriate assays, imaging, or molecular analysis. Researchers interpret substrate use and energy-related changes in relation to workload, oxygen availability, and disease state. This workflow can connect altered metabolism with outcomes such as ischemia, heart failure, diabetes, or hypertrophy.
This approach is especially useful when researchers need to connect altered energy handling with cardiovascular disease or therapeutic development. Findings may support biomarker discovery in conditions such as ischemia, heart failure, diabetes, and hypertrophy. They can also guide efforts to improve energy production or restore metabolic flexibility, providing a metabolic perspective on disease mechanisms and treatment strategies.