Intestinal Mucosa Contact
Intestinal Mucosa Contact - Related Videos
06:38
A Novel Method for the Culture and Polarized Stimulation of Human Intestinal Mucosa Explants
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Cited by 16 •
2013
We introduce a novel method for the maintenance of human intestinal mucosa in culture and monitoring of the response to various types of stimuli over at least 24 hrs. With our method, the polarity of the tissue is maintained, allowing for a physiological stimulation via the apical route.
08:54
Development of a Multicellular Three-dimensional Organotypic Model of the Human Intestinal Mucosa Grown Under Microgravity
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Cited by 20 •
2016
Cells growing in a three-dimensional (3-D) environment represent a marked improvement over cell cultivation in 2-D environments (e.g., flasks or dishes). Here we describe the development of a multicellular 3-D organotypic model of the human intestinal mucosa cultured under microgravity provided by rotating-wall-vessel (RWV) bioreactors.
04:46
Isolation of Lymphocytes from Mouse Genital Tract Mucosa
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Cited by 10 •
2012
An efficient way to isolate lymphocytes from mouse genital tract is described. This method takes advantage of enzyme digestion and Percoll gradient separation to allow efficient isolation. This technique is also adaptable to for use in other...
06:16
Cryosectioning Method for Microdissection of Murine Colonic Mucosa
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Cited by 5 •
2015
Here we describe a simple method for the isolation of spatially distinct murine colonic epithelial surface cells from the underlying crypt-base cells.
10:58
A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids
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Cited by 81 •
2015
In this video protocol we give a step by step explanation of lentiviral transduction in organoids of primary intestinal epithelium and of processing and downstream analysis of these cultures by quantitative RT-PCR, RNA-microarray and immunohistochemistry.
FAQs
The outcome depends on what happens after a compound reaches the mucosal surface. Diffusion can move it through the local environment, transport can influence passage across epithelial cells, and binding can retain it near particular components. These processes help determine whether the substance is absorbed, remains locally active, or contributes to a barrier response.
These structures create distinct sites of interaction rather than a single uniform surface. Mucus can affect how closely a substance approaches epithelial cells, while epithelial cells and underlying tissue influence transport and exposure. Immune components add a protective dimension, helping studies consider both intended activity and possible responses to luminal substances.
The result depends on how a substance interacts with the mucosal interface and how far it progresses beyond the lumen. Diffusion, transport, and binding can shape its location, while barrier responses may limit or modify exposure. Assessing these outcomes helps distinguish useful absorption or local action from unwanted irritation and mucosal safety concerns.
A study can examine the interaction between a substance and the mucosal lining in relation to several outcomes: movement toward epithelial cells, passage associated with absorption, activity at the local interface, and possible irritation. Considering these outcomes together provides a broader assessment than evaluating absorption alone, particularly for oral medicines and delivery systems.
The intestinal interface can determine whether an orally administered compound reaches epithelial cells, crosses into underlying tissue, or acts locally. This makes contact relevant to designing oral drugs, nutritional therapies, and delivery systems. Researchers can use the interaction to consider intended delivery, absorption, local effects, and the safety of the mucosal lining.
Studying contact with the mucosal lining connects luminal exposure to changes in permeability, host defense, and disease-related mechanisms. It helps researchers examine how substances interact with epithelial and immune components and whether barrier behavior changes. In medicine, this context supports investigations of gastrointestinal disorders as well as therapies intended to protect or influence the mucosa.